Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activator of cholesterol side-chain cleavage isolated from the cytosol of bovine adrenal cortex | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cholesterol | inhibition above 0.003 mM, mitochondrial | Bos taurus | |
Cholesterol sulfate | inhibition above 0.005 mM, mitochondrial | Bos taurus | |
additional information | no substrate inhibition with cytosolic enzyme, inhibitor of cholesterol-side-chain cleavage isolated from the cytosol of bovine adrenal cortex | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
Cholesterol sulfate | mitochondrial | Bos taurus | |
0.0005 | - |
cholesterol | mitochondrial | Bos taurus | |
0.0165 | - |
cholesterol | cytosolic | Bos taurus | |
0.0232 | - |
Cholesterol sulfate | cytosolic | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
mitochondrial inner membrane | inner membrane | Bos taurus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | isocaproic aldehyde additional product | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
column chromatography, gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | isocaproic aldehyde additional product | ? | |
cholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
cholesterol sulfate + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone sulfate + 17-hydroxy-pregnenolone + dehydroisoandrosterone sulfate + oxidized adrenodoxin + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
NADPH | NADPH-linked adrenodoxin reductase | Bos taurus |