Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | 5-20% concentration, 20-50% increase of enzyme activity | Bos taurus | |
Lipid | from adrenal mitochondria accelerates activity | Bos taurus |
General Stability | Organism |
---|---|
glycerol, 50%, stabilizes | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
20alpha-hydroxycholesterol | - |
Bos taurus | |
0.19 | - |
cholesterol | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | electron carriers and P-450 liquid-bound within mitochondrion | Bos taurus | 5743 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
800000 | - |
exclusion chromatography | Bos taurus |
850000 | - |
sedimentation equilibrium analysis | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + 6 reduced adrenodoxin + 3 O2 | Bos taurus | first step in biosynthesis of all steroid hormones | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cholic acid extraction, ammonium sulfate precipitation, DEAE-cellulose, hydroxylapatite and gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.0, 50% glycerol, 3 months | Bos taurus |
5°C, 50 mM potassium phosphate buffer, pH 7.0, without glycerol, 21-43% decrease of activity within 30 days, completely lost in 4 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
20alpha-hydroxycholesterol + reduced adrenodoxin + O2 | - |
Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
cholesterol + 6 reduced adrenodoxin + 3 O2 | first step in biosynthesis of all steroid hormones | Bos taurus | pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O | - |
? | |
cholesterol + reduced adrenodoxin + O2 | without demonstrable accumulation of putative intermediates 20alpha-hydroxycholesterol and 20alpha,22-dehydroxycholesterol | Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
for cholesterol and 20alpha-hydroxycholesterol, 75 mM potassium phosphate buffer | Bos taurus |
7 | - |
assay at | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | less than 50% of maximal activity below and above range | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
heme | 8 heme groups per molecule P-450 | Bos taurus | |
NADPH | - |
Bos taurus |