Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij | 56, 76 and 96 | Bos taurus | |
Emulgen | 911 and 913 | Bos taurus | |
Triton X-100 | effect onto P-450 itself suggested | Bos taurus | |
Tween 20 | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Bos taurus | 5743 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cholate extraction, pyrophosphate treatment, affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, for months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + reduced adrenodoxin + O2 | 20- or 22-hydroxycholesterol and 20,22-dehydroxycholesterol are putative intermediates | Bos taurus | pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O | - |
? | |
additional information | one-enzyme-three-step hypothesis | Bos taurus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adrenodoxin | - |
Bos taurus | |
NADPH | - |
Bos taurus |