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Literature summary for 1.14.15.6 extracted from

  • Nakajin, S.; Ishii, Y.; Shinoda, M.
    Binding of Triton X-100 to purified cytochrome P-450scc and enhancement of the cholesterol side chain cleavage activity (1979), Biochem. Biophys. Res. Commun., 87, 524-531.
    View publication on PubMed
Search for more literature for 1.14.15.6

Activating Compound

Activating Compound Comment Organism Structure
Brij 56, 76 and 96 Bos taurus
Emulgen 911 and 913 Bos taurus
Triton X-100 effect onto P-450 itself suggested Bos taurus
Tween 20
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
 Bos taurus 5743
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cholate extraction, pyrophosphate treatment, affinity chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, for months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + reduced adrenodoxin + O2 20- or 22-hydroxycholesterol and 20,22-dehydroxycholesterol are putative intermediates Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
additional information one-enzyme-three-step hypothesis Bos taurus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
adrenodoxin
-
 Bos taurus
NADPH
-
 Bos taurus