General Stability | Organism |
---|---|
11-deoxycortisol, partially stabilizes | Curvularia lunata |
glycerol, partially stabilizes | Curvularia lunata |
GSH, partially stabilizes | Curvularia lunata |
PMSF, partially stabilizes | Curvularia lunata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | strongly | Curvularia lunata | |
HgCl2 | 0.2 mM, 50% inhibition | Curvularia lunata | |
ketoconazole | - |
Curvularia lunata | |
Metyrapone | - |
Curvularia lunata | |
PCMB | 0.1 mM, 50% inhibition | Curvularia lunata | |
sodium cholate | 0.2% effect nearly 20% inhibition | Curvularia lunata | |
Stilbestrol | - |
Curvularia lunata | |
sulfhydryl reagents | strongly | Curvularia lunata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | lipids such as DLPC required | Curvularia lunata | |
0.077 | - |
11-deoxycortisol | 11beta-hydroxylation | Curvularia lunata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Curvularia lunata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia lunata | - |
Boedijn NRRL 2380 | - |
Purification (Comment) | Organism |
---|---|
Triton X-100, sodium cholate, column chromatographies | Curvularia lunata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | one bifunctional cytochrome P-450, 11beta-/14alpha-hydroxylation | Curvularia lunata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Curvularia lunata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
dependent on DLPC concentration, with 0.05 mM maximum activity, 2fold increase with addition of 15% glycerol, 0.6 mM GSH, 0.01 mM FAD and 0.01 mM FMN | Curvularia lunata |
Storage Stability | Organism |
---|---|
-80°C, purified, 200 mM sodium phosphate buffer, pH 8.0, 0.2 mM 11-deoxycortisol, 20% glycerol, 0.1 mM GSH, 0.1% sodium cholate, several days without loss of activity | Curvularia lunata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycortisol + reduced adrenal ferredoxin + O2 | 11beta-hydroxylase activity | Curvularia lunata | cortisol + oxidized adrenal ferredoxin + H2O | - |
? | |
11-deoxycortisol + reduced adrenal ferredoxin + O2 | 14alpha-hydroxylase activity | Curvularia lunata | 14alpha-hydroxy-11-deoxycortisol + oxidized adrenal ferredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Curvularia lunata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30 mM sodium phosphate buffer | Curvularia lunata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min, 30 mM sodium phosphate buffer, pH 8.0, decrease to 30%, improved to 45-70% by addition of 1 mM GSH, 0.2 mM 11-deoxycortisol, 0.5 mM PMSF or 20% glycerol | Curvularia lunata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.45 | - |
11-deoxycortisol | 11beta-hydroxylation | Curvularia lunata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
30 mM sodium phosphate buffer | Curvularia lunata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH-linked cytochrome P-450 reductase | Curvularia lunata |