Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme, encoded by gene camC, with gene camB, encoding the putidaredoxin, in Escherichia coli strains BL21(DE3), expression of mutant enzymes in Escherichia coli strain DH10B | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
I396A | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
I396G | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
I396V | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
M395I | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
M96Y | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
additional information | establishment of an in vitro screening system for P450cam selecting variants by the activity on NADH | Pseudomonas putida |
N244L | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
T297D | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
V87F | site-directed mutagenesis, the substrate specificity is altered compared to the wild-type enzyme | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-camphor + putidaredoxin + O2 | Pseudomonas putida | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(1R)-camphor + putidaredoxin + O2 | Pseudomonas putida PpG1 | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
gene camC | - |
Pseudomonas putida PpG1 | - |
gene camC | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cells grown in D-camphor minimal medium | Pseudomonas putida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | Pseudomonas putida | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam | Pseudomonas putida | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam in a coupled assay method | Pseudomonas putida PpG1 | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(1R)-camphor + putidaredoxin + O2 | putidaredoxin transfers electrons from NADH to P450cam | Pseudomonas putida PpG1 | 5-exo-(1R)-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
additional information | substrate specificities of wild-type and mutant enzymes, overview | Pseudomonas putida | ? | - |
? | |
additional information | substrate specificities of wild-type and mutant enzymes, overview | Pseudomonas putida PpG1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP101 | - |
Pseudomonas putida |
P450cam | - |
Pseudomonas putida |
P450cam monooxygenase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
putidaredoxin | transferring electrons from NADH to P450cam in a coupled assay method | Pseudomonas putida |