Literature summary for 1.14.15.1 extracted from

Hayashi, T.; Harada, K.; Sakurai, K.; Shimada, H.; Hirota, S.
A role of the heme-7-propionate side chain in cytochrome P450cam as a gate for regulating the access of water molecules to the substrate-binding site (2009), J. Am. Chem. Soc., 131, 1398-1400.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
the reconstituted P450cam at 1.8 A resolution reveals that the asymmetric one-legged heme is incorporated into the heme pocket in the same plane and in essentially the same conformation as the heme of the wild-type. A unique array of water molecules extending from the Tyr96 residue to the outside of the protein are present in the crystal structure	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	removal of heme-7-propionate decreases the (+)-camphor affinity by approximately 3fold, but exerts less of an influence on the other steps of the catalytic cycle of the monooxygenation reaction catalyzed by P450cam, does not exert an influence on the structure and electronic properties of the heme	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	purified reconstituted P450cam exhibits the ferrous CO-bound P450cam spectrum with the characteristic Soret band at 446 nm, indicating that the thiolate of Cys357 is ligated to the heme iron of the one-legged heme as seen in the wild-type protein	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	P00183	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-camphor + O2 + reduced putidaredoxin	the 7-propionate side chain plays a role in maintaining the high affinity of cytochrome P450cam for its substrate, the Asp297 and Gln322 residues are capable of undergoing a 7-propionate-associated conformational change in the protein interior	Pseudomonas putida	exo-5-hydroxycamphor + oxidized putidaredoxin + H2O	-	?

Synonyms

Synonyms	Comment	Organism
cytochrome p450cam	-	Pseudomonas putida
P450cam	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
putidaredoxin	-	Pseudomonas putida

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Release 2023.1 (January 2023)