Application | Comment | Organism |
---|---|---|
analysis | the enzyme is useful in whole cell biocatalyst systems | Pseudomonas putida |
synthesis | the enzyme is useful in whole cell biocatalyst systems | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expression of enzyme and mutant cofactor in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a catalytically active recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system from Pseudomonas putida coupled with enzymatic cofactor putidaredoxin mutant C73S/C85S regeneration, the cofactor mutant is 2fold more effective than the wild-type putidaredoxin | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + O2 + reduced putidaredoxin | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(+)-camphor + O2 + reduced putidaredoxin | regio- and stereospecific hydroxylation | Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome p450cam | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo activity | Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature profile | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
in vivo and in vitro pH optimum | Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | is required for O2 activation | Pseudomonas putida | |
putidaredoxin | wild-type and mutant C73S/C85S cofactor, the mutant shows about 2fold improved substrate conversion | Pseudomonas putida |