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Literature summary for 1.14.15.1 extracted from
- A recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system coupled with enzymatic cofactor regeneration (2006), Appl. Microbiol. Biotechnol., 72, 514-520.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | activates in vivo and in vitro | Pseudomonas putida |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional co-expression with glycerol dehydrogenase in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a facile way to significantly enhance the catalytic efficiency of the P450cam system by the coupling of its native electron transfer system with enzymatic NADH regeneration catalyzed by glycerol dehydrogenase in Escherichia coli whole cell biocatalysts, production of (+)-exo-5-hydroxycamphor and 5-keto-camphor, overview | Pseudomonas putida |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | as Fe2+-CO complex, the enzyme is a cytochrome P450 enzyme | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + O2 + reduced putidaredoxin | Pseudomonas putida | - |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytochrome P450cam monooxygenase | - |
Pseudomonas putida |
| P450cam | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| putidaredoxin | - |
Pseudomonas putida |
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