Protein Variants | Comment | Organism |
---|---|---|
T101M | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 89:11 | Pseudomonas putida |
T101M/T185F/V247M | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 87:13 | Pseudomonas putida |
T185F | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 78:22 | Pseudomonas putida |
T185L | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 80:20 | Pseudomonas putida |
T185V | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 74:26 | Pseudomonas putida |
V247A | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 87:13 | Pseudomonas putida |
V247M | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 83:17 | Pseudomonas putida |
V295I | ratio of (R)- to (S)-1-phenylethanol produced from ethylbenzene is 76:24 | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + putidaredoxin + O2 | Pseudomonas putida | - |
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
Accession Number PC00183 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + putidaredoxin + O2 | - |
Pseudomonas putida | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
ethylbenzene + putidaredoxin + O2 | at 5% of the reaction with (+)-camphor | Pseudomonas putida | 1-phenylethanol + oxidized putidaredoxin + H2O | ratio of (R)- to (S)-1-phenylethanol produced depends on mutant form | ? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas putida |