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Literature summary for 1.14.15.1 extracted from

  • Smith Eble, K.; Dawson, J.H.
    Novel reactivity of cytochrome P-450-CAM. Methyl hydroxylation of 5,5-difluorocamphor (1984), J. Biol. Chem., 259, 14389-14393.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pseudomonas putida 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ requirement, enzyme complex with two FeS-protein components Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-camphor + putidaredoxin + O2 Pseudomonas putida
-
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
Accession Number PC00183
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + putidaredoxin + O2
-
Pseudomonas putida (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O i.e. (+)-exo-5-hydroxycamphor ?
(+)-camphor + reduced putidaredoxin + O2
-
Pseudomonas putida (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O
-
r
5,5-difluorocamphor + putidaredoxin + O2
-
Pseudomonas putida 5,5-difluoro-9-hydroxycamphor + oxidized putidaredoxin + H2O
-
?
additional information
-
Pseudomonas putida ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.833
-
5,5-difluorocamphor cytochrome P-450 Pseudomonas putida
2.5
-
(+)-Camphor cytochrome P-450 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome m
-
Pseudomonas putida
NADH
-
Pseudomonas putida
putidaredoxin
-
Pseudomonas putida