Crystallization (Comment) | Organism |
---|---|
Pseudomonas putida PpG786, cytochrome m | Pseudomonas putida |
General Stability | Organism |
---|---|
2-mercaptoethanol retards loss of FeS-chromophore from putidaredoxin during purification | Pseudomonas putida |
glycerol, minimizes loss of FMN during purification of putidaredoxin reductase | Pseudomonas putida |
multiple freezing and thawing, at -20°C, cytochrome m accumulates an equally active, heme-containing component of higher molecular weight, DTT reconverts it to native cytochrome m at 25°C | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | requirement, enzyme complex with two FeS-protein components | Pseudomonas putida | |
K+ | increase of activity, selective effector of enhanced substrate affinity to cytochrome m | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
putidaredoxin reductase: MW 45000 Da, putidaredoxin: MW 11000 Da, cytochrome m: MW 44000 Da, Pseudomonas putida PpG786, gel filtration | Pseudomonas putida |
additional information | - |
putidaredoxin reductase, MW 43500 Da, putidaredoxin, MW 11594 Da, cytochrome m, MW 45000 Da, Pseudomonas putida PpG786, analytical data | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + putidaredoxin + O2 | Pseudomonas putida | - |
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
PpG786, mutant derived from PpG1 | - |
Pseudomonas putida | - |
Accession Number PC00183 | - |
Purification (Comment) | Organism |
---|---|
PpG786 | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.26 | - |
putidaredoxin reductase | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-196°C, no loss of activity of putidaredoxin reductase after repeated freeze-thaw cycles | Pseudomonas putida |
0°C, putidaredoxin slowly loses its prosthetic group, 2-mercaptoethanol retards apoprotein formation | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + putidaredoxin + O2 | - |
Pseudomonas putida | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? |
Subunits | Comment | Organism |
---|---|---|
More | multi-component mixed function monooxygenase consisting of putidaredoxin reductase, MW 47000, putidaredoxin, MW 11700, cytochrome m, MW 50500, Pseudomonas putida PpG786, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
putidaredoxin | constituent protein of the multi-component oxygenase | Pseudomonas putida | |
34 | - |
cytochrome m | P-450cam, constituent part of the multi-component enzyme | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome m | cytochrome P-450cam, essential, b-type heme-thiolate protein of P-450-class | Pseudomonas putida | |
FMN | - |
Pseudomonas putida | |
NADH | - |
Pseudomonas putida | |
putidaredoxin | essential, iron-sulfur redox protein, Fe2S2*Cys4-class, e-transfer agent to and effector of cytochrome P450cam | Pseudomonas putida |