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Literature summary for 1.14.14.9 extracted from

  • Guo, W.; Zhou, W.; Zhou, H.; Chen, X.
    Characterization of enzymatic properties of two novel enzymes, 3,4-dihydroxyphenylacetate dioxygenase and 4-hydroxyphenylacetate 3-hydroxylase, from Sulfobacillus acidophilus TPY (2019), BMC Microbiol., 19, 40 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfobacillus acidophilus

Organism

Organism UniProt Comment Textmining
Sulfobacillus acidophilus
-
-
-
Sulfobacillus acidophilus TPY
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.37
-
 coupled assay with reductase component HpaC and 3,4-dihydroxyphenylacetate dioxygenase, pH 8.0, 25°C Sulfobacillus acidophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylacetate + NADH + H+ + O2
-
 Sulfobacillus acidophilus 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?
4-hydroxyphenylacetate + NADH + H+ + O2
-
 Sulfobacillus acidophilus TPY 3,4-dihydroxyphenylacetate + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Sulfobacillus acidophilus

Synonyms

Synonyms Comment Organism
HpaB
-
 Sulfobacillus acidophilus
TPY_2462
-
 Sulfobacillus acidophilus

Cofactor

Cofactor Comment Organism Structure
additional information no electron donor: NADH Sulfobacillus acidophilus

General Information

General Information Comment Organism
metabolism presence of reductase component HpaC, EC 1.5.1.36, is absolutely required Sulfobacillus acidophilus