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Literature summary for 1.14.14.3 extracted from
- Role of Hsp70 (DnaK-DnaJ-GrpE) and Hsp100 (ClpA and ClpB) chaperones in refolding and increased thermal stability of bacterial luciferases in Escherichia coli cells (2002), Biochemistry (Moscow), 67, 986-992.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme shows enhanced affinity for chaperone ClpA | Photorhabdus luminescens | |
| additional information | the enzyme shows enhanced affinity for chaperone DnaK | Aliivibrio fischeri |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in different Escherichia coli strains, which are wild-type, or deficient in gene clpA, clpB, and clpX encoding Hsp chaperones, respectively | Aliivibrio fischeri |
| expression in different Escherichia coli strains, which are wild-type, or deficient in gene clpA, clpB, and clpX encoding Hsp chaperones, respectively | Photorhabdus luminescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RCHO + FMNH2 + O2 | Aliivibrio fischeri | 3-step process via H2O2 as intermediate | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir |  |
| RCHO + FMNH2 + O2 | Photorhabdus luminescens | 3-step process via H2O2 as intermediate | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aliivibrio fischeri | - |
marine bacterium | - |
| Photorhabdus luminescens | - |
terrestrial bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Aliivibrio fischeri |
| recombinant enzyme from Escherichia coli | Photorhabdus luminescens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| quick refolding within several min at room temperature or 25°C of thermoinactivated enzyme requires ATP and the DnaK-DnaJ-GrpE-system encoding the Hsp70 chaperone but is independent of chaperone ClpA, 80% activity after reactivation, refolding depends on the Escherichia coli strain used for recombinant expression of the luciferase, overview | Aliivibrio fischeri |
| slow refolding at room temperature or 35°C of thermoinactivated recominant enzyme requires the DnaK-DnaJ-GrpE-system encoding the Hsp70 chaperone, 7-8% activity after refolding over 10 min, refolding depends on the Escherichia coli strain used for recombinant expression of the luciferase, overview | Photorhabdus luminescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| n-decanal + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Aliivibrio fischeri | n-decanoate + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
| n-decanal + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Photorhabdus luminescens | n-decanoate + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
| RCHO + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Aliivibrio fischeri | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
| RCHO + FMNH2 + O2 | 3-step process via H2O2 as intermediate | Photorhabdus luminescens | RCOOH + FMN + H2O + hn | generation of blue-green light of wavelength 490 nm | ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacterial luciferase | - |
Aliivibrio fischeri |
| bacterial luciferase | - |
Photorhabdus luminescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Aliivibrio fischeri |
| 35 | - |
- |
Photorhabdus luminescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the recombinant enzyme shows reduced thermal stability in absence of chaperone Hsp100 after expression in an Escherichia coli clpA-mutant strain, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo | Photorhabdus luminescens |
| additional information | - |
the recombinant enzyme shows unaffected thermal stability after expression in an Escherichia coli clpA-mutant strain lacking chaperone Hsp100, carbonyl cyanide 3-chlorophenylhydrazone highly decreases the thermal stability of the enzyme in vivo to in vitro level, lack of chaperone ClpB decreases the thermal stability of the recombinant enzyme in vivo | Aliivibrio fischeri |
| 43.5 | - |
inactivation in vitro, temperature-stable enzyme | Photorhabdus luminescens |
| 43.5 | - |
inactivation, temperature-labile enzyme | Aliivibrio fischeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Aliivibrio fischeri |
| 7 | - |
assay at | Photorhabdus luminescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Aliivibrio fischeri | |
| FMNH2 | - |
Photorhabdus luminescens | |
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