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Literature summary for 1.14.14.3 extracted from

  • Hosseinkhani, S.; Szittner, R.; Meighen, E.A.
    Random mutagenesis of bacterial luciferase: critical role of Glu175 in the control of luminescence decay (2005), Biochem. J., 385, 575-580.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and randomly generated mutants in Escherichia coli strain BL21 Photorhabdus luminescens

Protein Variants

Protein Variants Comment Organism
D232G random mutagenesis, 63% of wild-type luminescence activity Photorhabdus luminescens
E175G random mutagenesis, the single point mutation leads to increased decay rate of the enzyme, 0.9% of wild-type luminescence activity Photorhabdus luminescens
E175G/N199D random mutagenesis, 0.1% of wild-type luminescence activity Photorhabdus luminescens
K202R random mutagenesis, 95% of wild-type luminescence activity Photorhabdus luminescens
M190T random mutagenesis, 29% of wild-type luminescence activity Photorhabdus luminescens
T198S random mutagenesis, 84% of wild-type luminescence activity Photorhabdus luminescens

General Stability

General Stability Organism
the wild-type enzyme belongs to the group of luciferases with slow decay, mutant E175G is turned into a luciferase with fast decay, the decay rate of the enzyme is determined by residue Glu175 Photorhabdus luminescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Photorhabdus luminescens the decay rate of the enzyme is determined by residue Glu175 of the central region of the LuxA subunit, distinction between slow and fast decay luciferases is primarily due to differences in aldehyde affinity and in the decomposition of the luciferase-flavin-oxygen intermediate ?
-
?
RCHO + FMNH2 + O2 Photorhabdus luminescens long-chain aldehydes RCOOH + FMN + H2O + hn long-chain fatty acids, bioluminescence reaction ir

Organism

Organism UniProt Comment Textmining
Photorhabdus luminescens
-
i.e. Photorhabdus luminescens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the decay rate of the enzyme is determined by residue Glu175 of the central region of the LuxA subunit, distinction between slow and fast decay luciferases is primarily due to differences in aldehyde affinity and in the decomposition of the luciferase-flavin-oxygen intermediate Photorhabdus luminescens ?
-
?
additional information substrate specificity and quantum yield of mutant E175G as a function of aldehyde chain length Photorhabdus luminescens ?
-
?
RCHO + FMNH2 + O2 long-chain aldehydes Photorhabdus luminescens RCOOH + FMN + H2O + hn long-chain fatty acids, bioluminescence reaction ir

Synonyms

Synonyms Comment Organism
bacterial luciferase
-
Photorhabdus luminescens
LuxAB
-
Photorhabdus luminescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Photorhabdus luminescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Photorhabdus luminescens

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Photorhabdus luminescens