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Literature summary for 1.14.14.3 extracted from

  • Szittner, R.; Jansen, G.; Thomas, D.Y.; Meighen, E.
    Bright stable luminescent yeast using bacterial luciferase as a sensor (2003), Biochem. Biophys. Res. Commun., 309, 66-70.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene luxAB, functional coexpression in Saccharomyces cerevisiae with NADPH-specific FMN reductase FRP from Vibrio harveyi, subcloning in Escherichia coli Vibrio harveyi

Protein Variants

Protein Variants Comment Organism
additional information Saccharomyces cerevisiae recombinantly expressing the Vibrio harveyi luciferase produces bright and stable luminescence, transformed yeast strains can grow on 0.5% v/v Z-9-tetradecenal, but die on 0.005% v/v decanal Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RCHO + FMNH2 + O2 Vibrio harveyi
-
RCOOH + FMN + H2O + hn
-
ir

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
gene luxA-B
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RCHO + FMNH2 + O2
-
Vibrio harveyi RCOOH + FMN + H2O + hn
-
ir

Synonyms

Synonyms Comment Organism
bacterial luciferase
-
Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Vibrio harveyi