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Literature summary for 1.14.14.1 extracted from

  • Schallmey, A.; Den Besten, G.; Teune, I.; Kembaren, R.; Janssen, D.
    Characterization of cytochrome P450 monooxygenase CYP154H1 from the thermophilic soil bacterium Thermobifida fusca (2011), Appl. Microbiol. Biotechnol., 89, 1475-1485.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43(DE3) cells Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography and HisTrap HP column chromatography Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-dimethoxybenzene + [reduced NADPH-hemoprotein reductase] + O2
-
 Thermobifida fusca ?
-
 ?
anisole + [reduced NADPH-hemoprotein reductase] + O2
-
 Thermobifida fusca ?
-
 ?
benzyl methyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 50% conversion Thermobifida fusca benzyl methyl sulfoxide + benzyl methyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 78:22 ?
ethyl phenyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 70% conversion Thermobifida fusca ethyl phenyl sulfoxide + ethyl phenyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 52:18 ?
ethylbenzene + [reduced NADPH-hemoprotein reductase] + O2
-
 Thermobifida fusca (R)-1-phenylethanol + 1-phenylethanone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 88:12 ?
indole + [reduced NADPH-hemoprotein reductase] + O2
-
 Thermobifida fusca (2Z)-2-(3-oxo-1,3-dihydro-2H-indol-2-ylidene)-1,2-dihydro-3H-indol-3-one + (3Z)-3-(3-oxo-1,3-dihydro-2H-indol-2-ylidene)-1,3-dihydro-2H-indol-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
methyl 4-methylphenyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 41% conversion Thermobifida fusca methyl 4-methylphenyl sulfoxide + methyl 4-methylphenyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 95:5 ?
methyl phenyl sulfide + O2 + NADH 55% conversion Thermobifida fusca methyl phenyl sulfoxide + methyl phenyl sulfone + NAD+ + H2O product ratio of 77:23 ?
additional information no activity with 3-acetylindole Thermobifida fusca ?
-
 ?
N-acetylindole + [reduced NADPH-hemoprotein reductase] + O2
-
 Thermobifida fusca 1-acetyl-1,2-dihydro-3H-indol-3-one + 1-acetyl-1,3-dihydro-2H-indol-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
propylbenzene + [reduced NADPH-hemoprotein reductase] + O2
-
 Thermobifida fusca (R)-1-phenyl-1-propanol + (R)-1-phenyl-1-propanol + 1-phenylpropan-1-one + [oxidized NADPH-hemoprotein reductase] + H2O product ratio of 79:7:14 ?
styrene + O2 + reduced putidaredoxin
-
 Thermobifida fusca (S)-styrene oxide + 2-phenylethanol + oxidized putidaredoxin + H2O product ratio of 81:19 ?
toluene + O2 + NADH
-
 Thermobifida fusca ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Thermobifida fusca

Synonyms

Synonyms Comment Organism
CYP154H1
-
 Thermobifida fusca
cytochrome P450 monooxygenase
-
 Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 67 the enzyme is moderately thermostable with an apparent melting temperature of 67°C and exhibits still 90% of initial activity after incubation at 50°C Thermobifida fusca