Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43(DE3) cells | Thermobifida fusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography and HisTrap HP column chromatography | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-dimethoxybenzene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Thermobifida fusca | ? | - |
? | |
anisole + [reduced NADPH-hemoprotein reductase] + O2 | - |
Thermobifida fusca | ? | - |
? | |
benzyl methyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 | 50% conversion | Thermobifida fusca | benzyl methyl sulfoxide + benzyl methyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O | product ratio of 78:22 | ? | |
ethyl phenyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 | 70% conversion | Thermobifida fusca | ethyl phenyl sulfoxide + ethyl phenyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O | product ratio of 52:18 | ? | |
ethylbenzene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Thermobifida fusca | (R)-1-phenylethanol + 1-phenylethanone + [oxidized NADPH-hemoprotein reductase] + H2O | product ratio of 88:12 | ? | |
indole + [reduced NADPH-hemoprotein reductase] + O2 | - |
Thermobifida fusca | (2Z)-2-(3-oxo-1,3-dihydro-2H-indol-2-ylidene)-1,2-dihydro-3H-indol-3-one + (3Z)-3-(3-oxo-1,3-dihydro-2H-indol-2-ylidene)-1,3-dihydro-2H-indol-2-one + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
methyl 4-methylphenyl sulfide + [reduced NADPH-hemoprotein reductase] + O2 | 41% conversion | Thermobifida fusca | methyl 4-methylphenyl sulfoxide + methyl 4-methylphenyl sulfone + [oxidized NADPH-hemoprotein reductase] + H2O | product ratio of 95:5 | ? | |
methyl phenyl sulfide + O2 + NADH | 55% conversion | Thermobifida fusca | methyl phenyl sulfoxide + methyl phenyl sulfone + NAD+ + H2O | product ratio of 77:23 | ? | |
additional information | no activity with 3-acetylindole | Thermobifida fusca | ? | - |
? | |
N-acetylindole + [reduced NADPH-hemoprotein reductase] + O2 | - |
Thermobifida fusca | 1-acetyl-1,2-dihydro-3H-indol-3-one + 1-acetyl-1,3-dihydro-2H-indol-2-one + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
propylbenzene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Thermobifida fusca | (R)-1-phenyl-1-propanol + (R)-1-phenyl-1-propanol + 1-phenylpropan-1-one + [oxidized NADPH-hemoprotein reductase] + H2O | product ratio of 79:7:14 | ? | |
styrene + O2 + reduced putidaredoxin | - |
Thermobifida fusca | (S)-styrene oxide + 2-phenylethanol + oxidized putidaredoxin + H2O | product ratio of 81:19 | ? | |
toluene + O2 + NADH | - |
Thermobifida fusca | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Thermobifida fusca |
Synonyms | Comment | Organism |
---|---|---|
CYP154H1 | - |
Thermobifida fusca |
cytochrome P450 monooxygenase | - |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 67 | the enzyme is moderately thermostable with an apparent melting temperature of 67°C and exhibits still 90% of initial activity after incubation at 50°C | Thermobifida fusca |