Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FMN | the linker region between P450 and FMN domain proves to be important for the effective biotransformation of (+)-camphor | synthetic construct |
Cloned (Comment) | Organism |
---|---|
chimeric oxygenase, in which the P450cam domain is fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784. Plasmids containing the P450cam-RhFRed gene (L1-L7) transformed into Escherichia coli BL21-DE3cells | synthetic construct |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
synthetic construct | - |
- |
- |
Purification (Comment) | Organism |
---|---|
in two steps by nickel chelating column chromatography followed by anion exchange column chromatography | synthetic construct |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + [reduced NADPH-hemoprotein reductase] + O2 | - |
synthetic construct | (+)-exo-5-hydroxycamphor + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P450cam | - |
synthetic construct |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
synthetic construct |
General Information | Comment | Organism |
---|---|---|
physiological function | chimeric P450cam-RhFRed reductase domain enzyme shows improved biotransformation of 80% conversion at 30 mM substrate concentration | synthetic construct |