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Literature summary for 1.14.14.1 extracted from

  • Robin, A.; Roberts, G.A.; Kisch, J.; Sabbadin, F.; Grogan, G.; Bruce, N.; Turner, N.J.; Flitsch, S.L.
    Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme (2009), Chem. Commun. (Camb. ), 0, 2478-2480.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
FMN the linker region between P450 and FMN domain proves to be important for the effective biotransformation of (+)-camphor synthetic construct

Cloned(Commentary)

Cloned (Comment) Organism
chimeric oxygenase, in which the P450cam domain is fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784. Plasmids containing the P450cam-RhFRed gene (L1-L7) transformed into Escherichia coli BL21-DE3cells synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct
-
-
-

Purification (Commentary)

Purification (Comment) Organism
in two steps by nickel chelating column chromatography followed by anion exchange column chromatography synthetic construct

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + [reduced NADPH-hemoprotein reductase] + O2
-
synthetic construct (+)-exo-5-hydroxycamphor + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
P450cam
-
synthetic construct

Cofactor

Cofactor Comment Organism Structure
NADPH
-
synthetic construct

General Information

General Information Comment Organism
physiological function chimeric P450cam-RhFRed reductase domain enzyme shows improved biotransformation of 80% conversion at 30 mM substrate concentration synthetic construct