Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is inducible by vanillic acid and veratric acid | Comamonas testosteroni |
Cloned (Comment) | Organism |
---|---|
gene ivaA, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli | Comamonas testosteroni |
gene ivaB, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli | Comamonas testosteroni |
gene vanA, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovanillic acid + NAD(P)H + H+ + O2 | Comamonas testosteroni | O-demethylation by IvaAB | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
isovanillic acid + NAD(P)H + H+ + O2 | Comamonas testosteroni BR6020 | O-demethylation by IvaAB | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
vanillic acid + NAD(P)H + H+ + O2 | Comamonas testosteroni | O-demethylation by VanA | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
vanillic acid + NAD(P)H + H+ + O2 | Comamonas testosteroni BR6020 | O-demethylation by VanA | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | Comamonas testosteroni | O-demethylation by VanA | isovanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | Comamonas testosteroni | O-demethylation by IvaAB | vanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | Comamonas testosteroni BR6020 | O-demethylation by IvaAB | vanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | Comamonas testosteroni BR6020 | O-demethylation by VanA | isovanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q2KQ78 | gene ivaB; genes ivaA, and ivaB | - |
Comamonas testosteroni | Q2KQ79 | gene ivaA; genes ivaA, and ivaB | - |
Comamonas testosteroni | Q2KQ82 | gene vanA; gene vanA | - |
Comamonas testosteroni BR6020 | Q2KQ78 | gene ivaB; genes ivaA, and ivaB | - |
Comamonas testosteroni BR6020 | Q2KQ79 | gene ivaA; genes ivaA, and ivaB | - |
Comamonas testosteroni BR6020 | Q2KQ82 | gene vanA; gene vanA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | in succinate- and procatechuic acid-grown cells, there is negligible degradative activity towards vanillic acid, veratric acid, and isovanillic acid and little to no expression of genes van, ivaA, and ivaB, growth on vanillic acid or veratric acid results in production of active oxygenases and expression of vanA, ivaA, and ivaB | Comamonas testosteroni | - |
additional information | in succinate- and procatechuic acid-grown cells, there is negligible degradative activity towards vanillic acid, veratric acid, and isovanillic acid and little to no expression of genes vanA, ivaA, and ivaB, growth on vanillic acid or veratric acid results in production of active oxygenases and expression of vanA, ivaA, and ivaB | Comamonas testosteroni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovanillic acid + NAD(P)H + H+ + O2 | O-demethylation by IvaAB | Comamonas testosteroni | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
isovanillic acid + NAD(P)H + H+ + O2 | i.e. 3-hydroxy-4-methoxybenzoate, O-demethylation by IvaAB | Comamonas testosteroni | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | procatechuic acid is 3,4-dihydroxybenzoate | ? | |
isovanillic acid + NAD(P)H + H+ + O2 | O-demethylation by IvaAB | Comamonas testosteroni BR6020 | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
isovanillic acid + NAD(P)H + H+ + O2 | i.e. 3-hydroxy-4-methoxybenzoate, O-demethylation by IvaAB | Comamonas testosteroni BR6020 | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | procatechuic acid is 3,4-dihydroxybenzoate | ? | |
vanillic acid + NAD(P)H + H+ + O2 | O-demethylation by VanA | Comamonas testosteroni | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
vanillic acid + NAD(P)H + H+ + O2 | i.e. 4-hydroxy-3-methoxybenzoate, O-demethylation by VanA | Comamonas testosteroni | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | procatechuic acid is 3,4-dihydroxybenzoate | ? | |
vanillic acid + NAD(P)H + H+ + O2 | O-demethylation by VanA | Comamonas testosteroni BR6020 | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
vanillic acid + NAD(P)H + H+ + O2 | i.e. 4-hydroxy-3-methoxybenzoate, O-demethylation by VanA | Comamonas testosteroni BR6020 | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | procatechuic acid is 3,4-dihydroxybenzoate | ? | |
veratric acid + NAD(P)H + H+ + O2 | O-demethylation by VanA | Comamonas testosteroni | isovanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | i.e. 3,4-dimethoxybenzoate, O-demethylation by VanA | Comamonas testosteroni | isovanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | O-demethylation by VanA | Comamonas testosteroni BR6020 | isovanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | i.e. 3,4-dimethoxybenzoate, O-demethylation by VanA | Comamonas testosteroni BR6020 | isovanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | O-demethylation by IvaAB | Comamonas testosteroni | vanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | i.e. 3,4-dimethoxybenzoate, O-demethylation by IvaAB | Comamonas testosteroni | vanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | O-demethylation by IvaAB | Comamonas testosteroni BR6020 | vanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? | |
veratric acid + NAD(P)H + H+ + O2 | i.e. 3,4-dimethoxybenzoate, O-demethylation by IvaAB | Comamonas testosteroni BR6020 | vanillic acid + NAD(P)+ + H2O + formaldehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | presence of both subunits is essential | Comamonas testosteroni |
Synonyms | Comment | Organism |
---|---|---|
IvaAB | - |
Comamonas testosteroni |
More | the enzyme belongs to the phthalate family oxygenases | Comamonas testosteroni |
VanA | - |
Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Comamonas testosteroni |