KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics for kidney and liver enzymes, overview | Rattus norvegicus | |
0.01 | - |
Methimazole | pH 7.6, 37°C, purified FMO3 from liver | Rattus norvegicus | |
0.04 | - |
Methimazole | pH 7.6, 37°C, purified FMO3 from kidney | Rattus norvegicus | |
0.31 | - |
seleno-L-methionine | pH 7.6, 37°C, purified FMO3 from liver | Rattus norvegicus | |
0.35 | - |
seleno-L-methionine | pH 7.6, 37°C, purified FMO3 from kidney | Rattus norvegicus | |
2.8 | - |
L-methionine | pH 7.6, 37°C, purified FMO3 from kidney | Rattus norvegicus | |
3.4 | - |
L-methionine | pH 7.6, 37°C, purified FMO3 from liver | Rattus norvegicus | |
4.3 | - |
S-allyl-L-cysteine | pH 7.6, 37°C, purified FMO3 from kidney | Rattus norvegicus | |
5.9 | - |
S-allyl-L-cysteine | pH 7.6, 37°C, purified FMO3 from liver | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, kidney enzyme, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q9EQ76 | male sprague-dawley rats | - |
Purification (Comment) | Organism |
---|---|
native FMO3 130fold from kidney microsomes | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
purified enzyme, substrate L-methionine | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + NADPH + H+ + O2 | stereochemistry, overview | Rattus norvegicus | methionine S-oxide + NADP+ + H2O | formation of 80% D-isomer | ? | |
methimazole + NADPH + H+ + O2 | i.e. N-methyl-2-mercaptoimidazole | Rattus norvegicus | methimazole S-oxide + NADP+ + H2O | - |
? | |
S-allyl-L-cysteine + NADPH + H+ + O2 | stereochemmistry, overview | Rattus norvegicus | ? + NADP+ + H2O | - |
? | |
seleno-L-methionine + NADPH + H+ + O2 | - |
Rattus norvegicus | seleno-L-methionine Se-oxide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, kidney enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
FMO3 | - |
Rattus norvegicus |
Met S-oxidase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | consensus binding sequence is Gly-Xaa-Gly-Xaa-Xaa-Gly | Rattus norvegicus | |
NADPH | consensus binding sequence is Gly-Leu-Gly-Asn-Ser-Gly | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | FMO3 plays an important role in kidney metabolism of xenobiotics containing sulfur and selenium atoms | Rattus norvegicus |