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Literature summary for 1.14.13.8 extracted from
- Characterization and modulation by drugs of sheep liver microsomal flavin monooxygenase activity (2005), Cell Biochem. Funct., 23, 245-251.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cholate | 29% activation at 0.5% concentration | Ovis aries |  |
| Emulgen 913 | 350% activation at 0.2%, 47% activation at 1.0% concentration | Ovis aries | |
| Triton X-100 | 271% activation at 0.1%, 159% activation at 0.3% concentration | Ovis aries | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chlorpromazine | competitive inhibition of methimazole oxidation at high concentrations | Ovis aries | |
| HgCl2 | IC50: 4.7 mM, liver microsomes | Ovis aries | |
| imipramine | competitive inhibition of methimazole oxidation at high concentrations | Ovis aries | |
| MgCl2 | IC50: 77.5 mM, liver microsomes | Ovis aries | |
| n-octylamine | IC50: 4 mM, liver microsomes | Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.118 | - |
Methimazole | pH 8.0, 37°C, liver microsomes | Ovis aries | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | isozyme FMO3 | Ovis aries | - |
- |
| nuclear envelope | - |
Ovis aries | 5635 | - |
| smooth endoplasmic reticulum | - |
Ovis aries | 5790 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Ovis aries | the enzyme is involved in detoxification, generally, metabolites produced by FMO-catalysed reactions are more hydrophilic and less toxic, and are easily excreted from the body | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
7-9-months-old Akkaraman sheep, isozyme FMO3 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | isozyme FMO3 | Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0038 | - |
liver microsomes, substrate methimazole | Ovis aries |
| 0.0061 | - |
liver microsomes, substrate methimazole in presence of 0.1% Triton X-100 | Ovis aries |
| 0.0107 | - |
liver microsomes, substrate imipramine | Ovis aries |
| 0.0123 | - |
liver microsomes, substrate chlorpromazine | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorpromazine + NADPH + O2 | liver microsomes | Ovis aries | ? | - |
? | |
| imipramine + NADPH + O2 | liver microsomes | Ovis aries | ? | - |
? | |
| methimazole + NADPH + O2 | liver microsomes | Ovis aries | ? | - |
? | |
| additional information | the enzyme is involved in detoxification, generally, metabolites produced by FMO-catalysed reactions are more hydrophilic and less toxic, and are easily excreted from the body | Ovis aries | ? | - |
? | |
| additional information | substrates are a wide range of nucleophilic nitrogen-, sulfur-, phosphorus-, and selenium heteroatom-containing chemicals, drugs, and agricultural agents | Ovis aries | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavin monooxygenase | - |
Ovis aries |
| FMO | - |
Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Ovis aries |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | temperature profile | Ovis aries |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
liver microsomes, enzyme inactivation after 20 days | Ovis aries |
| 37 | - |
liver microsomes, enzyme inactivation after 48 h | Ovis aries |
| 50 | - |
liver microsomes, enzyme inactivation after 1.5 h | Ovis aries |
| 65 | - |
liver microsomes, enzyme inactivation after 5 min | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ovis aries |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | pH profile | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | enzyme-bound | Ovis aries | |
| NADPH | - |
Ovis aries | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 4 | - |
IC50: 4 mM, liver microsomes | Ovis aries | n-octylamine | |
| 4.7 | - |
IC50: 4.7 mM, liver microsomes | Ovis aries | HgCl2 | |
| 77.5 | - |
IC50: 77.5 mM, liver microsomes | Ovis aries | MgCl2 | |
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