Crystallization (Comment) | Organism |
---|---|
crystal structure of mutant enzyme Y201F, Y385F, and N300D | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
N300D | mutation has profound effect on enzyme structure. The side chain of Asp300 moves away from the flavin, disrupting the interaction of the carboxamide group with the flavin O(2) atom, and the alpha-helix H10 that begins at residue 297 is displaced, altering its dipole interaction with the flavin ring | Pseudomonas aeruginosa |
Y201F | crystals differ from the wild-type enzyme at two surface positions, 228 and 249 | Pseudomonas aeruginosa |
Y385F | crystals differ from the wild-type enzyme at two surface positions, 228 and 249 | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? |