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Literature summary for 1.14.13.2 extracted from

  • Eppink, M.H.M.; Schreuder, H.A.; Van Berkel, W.J.H.
    Lys42 and Ser42 variants of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens reveal that Arg42 is essential for NADPH binding (1998), Eur. J. Biochem., 253, 194-201.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes R42S and R42K expressed in transformed Escherichia coli TG2 cells Pseudomonas fluorescens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal of the enzyme complexed with 4-hydroxybenzoate are obtained using the hanging-drop method Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
R42K low activity results from impaired binding of NADPH Pseudomonas fluorescens
R42S low activity results from impaired binding of NADPH Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
4-hydroxybenzoate wild-type enzyme Pseudomonas fluorescens
0.05
-
NADPH wild-type enzyme Pseudomonas fluorescens
0.075
-
4-hydroxybenzoate mutant enzyme R42S Pseudomonas fluorescens
0.11
-
4-hydroxybenzoate mutant enzyme R42K Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzoate + NADPH + O2 Pseudomonas fluorescens enzyme catalyzes an intermediate step in the degradation of aromatic compounds in soil microorganisms ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
?
4-hydroxybenzoate + NADPH + O2 enzyme catalyzes an intermediate step in the degradation of aromatic compounds in soil microorganisms Pseudomonas fluorescens ?
-
?
additional information Arg42 is involved in binding of the 2'-phosphoadenosine moiety of NADPH Pseudomonas fluorescens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Pseudomonas fluorescens