Cloned (Comment) | Organism |
---|---|
mutant enzymes R42S and R42K expressed in transformed Escherichia coli TG2 cells | Pseudomonas fluorescens |
Crystallization (Comment) | Organism |
---|---|
crystal of the enzyme complexed with 4-hydroxybenzoate are obtained using the hanging-drop method | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
R42K | low activity results from impaired binding of NADPH | Pseudomonas fluorescens |
R42S | low activity results from impaired binding of NADPH | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
4-hydroxybenzoate | wild-type enzyme | Pseudomonas fluorescens | |
0.05 | - |
NADPH | wild-type enzyme | Pseudomonas fluorescens | |
0.075 | - |
4-hydroxybenzoate | mutant enzyme R42S | Pseudomonas fluorescens | |
0.11 | - |
4-hydroxybenzoate | mutant enzyme R42K | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | Pseudomonas fluorescens | enzyme catalyzes an intermediate step in the degradation of aromatic compounds in soil microorganisms | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas fluorescens | protocatechuate + NADP+ + H2O | - |
? | |
4-hydroxybenzoate + NADPH + O2 | enzyme catalyzes an intermediate step in the degradation of aromatic compounds in soil microorganisms | Pseudomonas fluorescens | ? | - |
? | |
additional information | Arg42 is involved in binding of the 2'-phosphoadenosine moiety of NADPH | Pseudomonas fluorescens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas fluorescens |