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Literature summary for 1.14.13.2 extracted from

  • Entsch, B.; Ballou, D.P.
    Purification, properties, and oxygen reactivity of p-hydroxybenzoate hydroxylase from Pseudomonas aeruginosa [published erratum appears in Biochim Biophys Acta 1990 Mar 29;1038(1):139] (1989), Biochim. Biophys. Acta, 999, 313-322.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0109
-
 4-hydroxybenzoate
-
 Pseudomonas aeruginosa
0.0232
-
 NADPH
-
 Pseudomonas aeruginosa
0.0371
-
 O2
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Pseudomonas aeruginosa
75000
-
 gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas aeruginosa protocatechuate + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 pH 5.5-8.5, stable Pseudomonas aeruginosa
40
-
 pH 8.0, stable Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.1 HEPES or Tris-SO42- buffer Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 mol of FAD per mol of enzyme Pseudomonas aeruginosa
NADPH
-
 Pseudomonas aeruginosa