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Literature summary for 1.14.12.12 extracted from

  • Barriault, D.; Sylvestre, M.
    Functionality of biphenyl 2,3-dioxygenase components in naphthalene 1,2-dioxygenase (1999), Appl. Microbiol. Biotechnol., 51, 592-597.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of histidine tagged enzyme in Escherichia coli Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 53000 + x * 25000, histidine-tagged recombinant enzyme, SDS-PAGE Pseudomonas putida
53000
-
x * 53000 + x * 25000, histidine-tagged recombinant enzyme, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida G7
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorobiphenyl + NADH + O2 recombinant histidine-tagged terminal dioxygenase Pseudomonas putida 2-chloro-2',3'-dihydrobiphenyl-2',3'-diol + NAD+
-
?
2-chlorobiphenyl + NADH + O2 recombinant histidine-tagged terminal dioxygenase Pseudomonas putida G7 2-chloro-2',3'-dihydrobiphenyl-2',3'-diol + NAD+
-
?
3-chlorobiphenyl + NADH + O2 recombinant histidine-tagged terminal dioxygenase Pseudomonas putida 3-chloro-2',3'-dihydrobiphenyl-2',3'-diol + NAD+
-
?
3-chlorobiphenyl + NADH + O2 recombinant histidine-tagged terminal dioxygenase Pseudomonas putida G7 3-chloro-2',3'-dihydrobiphenyl-2',3'-diol + NAD+
-
?
4-chlorobiphenyl + NADH + O2 recombinant histidine-tagged terminal dioxygenase Pseudomonas putida 4-chloro-2',3'-dihydrobiphenyl-2',3'-diol + NAD+
-
?
4-chlorobiphenyl + NADH + O2 recombinant histidine-tagged terminal dioxygenase Pseudomonas putida G7 4-chloro-2',3'-dihydrobiphenyl-2',3'-diol + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 53000 + x * 25000, histidine-tagged recombinant enzyme, SDS-PAGE Pseudomonas putida