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Literature summary for 1.14.11.4 extracted from

  • Gilkes, D.M.; Bajpai, S.; Wong, C.C.; Chaturvedi, P.; Hubbi, M.E.; Wirtz, D.; Semenza, G.L.
    Procollagen lysyl hydroxylase 2 is essential for hypoxia-induced breast cancer metastasis (2013), Mol. Cancer Res., 11, 456-466.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.4

Application

Application Comment Organism
diagnostics PLOD expression is associated with human breast cancer progression, PLOD2 expression is specifically prognostic in breast cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
genes PLOD1 and PLOD2, real-time quantitative reverse transcription PCR Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine-[procollagen] + 2-oxoglutarate + O2 Homo sapiens PLOD2 specifically hydroxylates lysines in the telopeptide of procollagens, whereas PLOD1 is responsible for lysine hydroxylation in the alpha-helical or central domain (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 genes PLOD1 and PLOD2
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Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
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 Homo sapiens
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lung
-
 Homo sapiens
-
lymph node
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
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MDA-MB-435 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine-[procollagen] + 2-oxoglutarate + O2
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 Homo sapiens (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
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 ?
L-lysine-[procollagen] + 2-oxoglutarate + O2 PLOD2 specifically hydroxylates lysines in the telopeptide of procollagens, whereas PLOD1 is responsible for lysine hydroxylation in the alpha-helical or central domain Homo sapiens (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
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 ?

Synonyms

Synonyms Comment Organism
lysyl hydroxylase 2
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens hypoxia-inducible factor 1 activates transcription of the PLOD1 and PLOD2 genes in hypoxic breast cancer cells, knockdown of HIF-1a expression blocks PLOD1 and PLOD2 induction under hypoxic conditions up

General Information

General Information Comment Organism
malfunction PLOD2 knockdown prevents fibrillar collagen formation in breast tumors, decreased PLOD2 expression in breast cancer cells inhibits metastasis, also lung metastasis is significantly impaired by PLOD2 knockdown Homo sapiens
physiological function in hypoxic breast cancer cells, hypoxia-inducible factor 1 activates transcription of the PLOD1 and PLOD2 genes encoding procollagen lysyl hydroxylases that are required for the biogenesis of collagen, which is a major constituent of the extracellular matrix. High PLOD2 expression in breast cancer biopsies is associated with increased risk of mortality. PLOD2 is critical for fibrillar collagen formation by breast cancer cells, increases tumor stiffness, and is required for metastasis to lymph nodes and lungs Homo sapiens