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Literature summary for 1.14.11.4 extracted from

  • Tsukamoto, H.; Yokoyama, Y.; Suzuki, T.; Mizuta, S.; Yoshinaka, R.; Akahane, Y.
    Cloning and expression of cDNA encoding lysyl hydroxylase 1, 2 and 3 in tiger puffer Takifugu rubripes (2013), Comp. Biochem. Physiol. B, 166, 123-132.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene encoding isozyme LH1, DNA and amino acid sequence determination and analysis, quantitative expression analysis Takifugu rubripes
gene encoding isozyme LH2a, DNA and amino acid sequence determination and analysis, quantitative expression analysis Takifugu rubripes
gene encoding isozyme LH3, DNA and amino acid sequence determination and analysis, quantitative expression analysis Takifugu rubripes

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information isozyme LH1 contains a signal peptide comprising residues Met1-Cys20 Takifugu rubripes
-
-
additional information isozyme LH2a contains a signal peptide comprising residues Met1-Ser19 Takifugu rubripes
-
-
additional information isozyme LH3 contains a signal peptide comprising residues Met1-Ala21 Takifugu rubripes
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84385
-
x * 84385, isozyme LH1, sequence calculation Takifugu rubripes
84558
-
x * 84558, isozyme LH3, sequence calculation Takifugu rubripes
85063
-
x * 85063, isozyme LH2a, sequence calculation Takifugu rubripes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine-[procollagen] + 2-oxoglutarate + O2 Takifugu rubripes
-
(2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Takifugu rubripes A5A6S1 LH1
-
Takifugu rubripes A5A6S2 LH3
-
Takifugu rubripes A5A6S3 LH2a
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isozyme LH1 contains 4 glycosylation sites Takifugu rubripes
glycoprotein isozyme LH2a contains 7 glycosylation sites Takifugu rubripes
glycoprotein isozyme LH3 contains 4 glycosylation sites Takifugu rubripes

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Takifugu rubripes
-
dorsal fin
-
Takifugu rubripes
-
embryo tissue expression profiles of the three isozymes are different, quantitative expression analysis, in situ localizations, overview Takifugu rubripes
-
eye
-
Takifugu rubripes
-
gill
-
Takifugu rubripes
-
heart
-
Takifugu rubripes
-
intestine
-
Takifugu rubripes
-
kidney head and body Takifugu rubripes
-
larva in the hatching larvae, LH1 is expressed in the pectoral fin bud Takifugu rubripes
-
larva in the hatching larvae, LH3 is expressed in the pectoral fin bud Takifugu rubripes
-
liver
-
Takifugu rubripes
-
muscle ordinary and dark Takifugu rubripes
-
muscle ordinary and dark, high expression of lysine hydroxylase 1 Takifugu rubripes
-
ovary
-
Takifugu rubripes
-
ovary high expression of lysine hydroxylase 3 Takifugu rubripes
-
skin
-
Takifugu rubripes
-
spleen
-
Takifugu rubripes
-
swim bladder
-
Takifugu rubripes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine-[procollagen] + 2-oxoglutarate + O2
-
Takifugu rubripes (2S,5R)-5-hydroxy-L-lysine-[procollagen] + succinate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 84385, isozyme LH1, sequence calculation Takifugu rubripes
? x * 84558, isozyme LH3, sequence calculation Takifugu rubripes
? x * 85063, isozyme LH2a, sequence calculation Takifugu rubripes

Synonyms

Synonyms Comment Organism
LH1
-
Takifugu rubripes
LH2a
-
Takifugu rubripes
LH3
-
Takifugu rubripes
lysyl hydroxylase 1
-
Takifugu rubripes
lysyl hydroxylase 2a
-
Takifugu rubripes
lysyl hydroxylase 3
-
Takifugu rubripes

pI Value

Organism Comment pI Value Maximum pI Value
Takifugu rubripes isozyme LH1, sequence calculation
-
5.8
Takifugu rubripes isozyme LH3, sequence calculation
-
6.1
Takifugu rubripes isozyme LH2a, sequence calculation
-
6.2

General Information

General Information Comment Organism
physiological function lysyl hydroxylase (LH) catalyzes the hydroxylation of lysine residues in collagens, and contributes to the formation of more stable collagen cross-links Takifugu rubripes