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Literature summary for 1.14.11.17 extracted from

  • Knauer, S.H.; Hartl-Spiegelhauer, O.; Schwarzinger, S.; Haenzelmann, P.; Dobbek, H.
    The Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers (2012), FEBS J., 279, 816-831.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of TauD in Escherichia coli strains BL21(DE3) and Rosetta Blue(DE3) Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
three crystal structures of the apo form, vapor diffusion techniques, protein solution contains 1. 27-28 m/ml TauDPp in 25 mM Tris-HCl, pH 7.7, or 2. 21 mg/ml TauDPp in 25 mM Tris-HCl pH 7.7, containing 10 mM taurine, 0.1 M (NH4)2SO4 and 20% v?v glycerol, mixed with reservoir solution containing 1. 15% w?v PEG 1000, 40% v?v PEG 400, 0.15 M NaK phosphate, and 0.1 M imidazole chloride, pH 6.5, or 2. 20% w?v PEG 5000 monomethyl ether, 0.1 M Bis-Tris-HCl, pH 6.5, X-ray diffraction structure determination and analysis at 1.85-2.6 A resolution Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on, mononuclear non-heme iron center, binding structure and kinetics, spectral analysis, overview Escherichia coli
Fe2+ dependent on, mononuclear non-heme iron center, binding structure and kinetics, spectral analysis, overview Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
4 * 31000, about, sequence calculation, TauDPp is a tetramer in solution and in the crystals Pseudomonas putida
32000
-
4 * 32000, about, sequence calculation, TauDEc is a tetramer in solution and in the crystals, gel filtration and sedimentation velocity analytical ultracentrifugation Escherichia coli
110000
-
gel filtration Escherichia coli
112000
-
gel filtration Pseudomonas putida
120000
-
sedimentation velocity analytical ultracentrifugation Pseudomonas putida
127000
-
sedimentation velocity analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taurine + 2-oxoglutarate + O2 Escherichia coli
-
sulfite + aminoacetaldehyde + succinate + CO2
-
?
taurine + 2-oxoglutarate + O2 Pseudomonas putida
-
sulfite + aminoacetaldehyde + succinate + CO2
-
?
taurine + 2-oxoglutarate + O2 Pseudomonas putida KT 2240
-
sulfite + aminoacetaldehyde + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas putida Q88RA3
-
-
Pseudomonas putida KT 2240 Q88RA3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.56
-
purified enzyme, pH and temperature not specified in the publication Escherichia coli
8.11
-
purified enzyme, pH and temperature not specified in the publication Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
taurine + 2-oxoglutarate + O2
-
Escherichia coli sulfite + aminoacetaldehyde + succinate + CO2
-
?
taurine + 2-oxoglutarate + O2
-
Pseudomonas putida sulfite + aminoacetaldehyde + succinate + CO2
-
?
taurine + 2-oxoglutarate + O2
-
Pseudomonas putida KT 2240 sulfite + aminoacetaldehyde + succinate + CO2
-
?

Subunits

Subunits Comment Organism
More topology of an apo-TauDPp monomer,overview Pseudomonas putida
tetramer 4 * 31000, about, sequence calculation, TauDPp is a tetramer in solution and in the crystals Pseudomonas putida
tetramer 4 * 32000, about, sequence calculation, TauDEc is a tetramer in solution and in the crystals, gel filtration and sedimentation velocity analytical ultracentrifugation Escherichia coli

Synonyms

Synonyms Comment Organism
Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenase
-
Escherichia coli
Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenase
-
Pseudomonas putida
TauD
-
Escherichia coli
TauD
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information real-time NMR spectra to follow enzymatic turnover Pseudomonas putida

General Information

General Information Comment Organism
additional information metal-to-ligand charge-transfer transition involving the 2-oxoglutarate ligand with a six-coordinate metal center. Taurine does not coordinate the Fe(II) ion, but binds in its close vicinity, leading to loss of the coordinating water molecule. This change of the six-coordinate to five-coordinate Fe(II) center causes a small perturbation of the absorption spectrum Escherichia coli
additional information metal-to-ligand charge-transfer transition involving the 2-oxoglutarate ligand with a six-coordinate metal center. Taurine does not coordinate the Fe(II) ion, but binds in its close vicinity, leading to loss of the coordinating water molecule. This change of the six-coordinate to five-coordinate Fe(II) center causes a small perturbation of the absorption spectrum Pseudomonas putida