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Literature summary for 1.14.11.17 extracted from

  • Matsuda, M.; Asano, Y.
    A simple assay of taurine concentrations in food and biological samples using taurine dioxygenase (2012), Anal. Biochem., 427, 121-123.
    View publication on PubMed

Application

Application Comment Organism
analysis development of a colorimetric assay method for determination of taurine in commercially available beverages and some biological samples using the taurine dioxygenase. Taurine determination in food control, biological research, and diagnoses based on urinary taurine concentration Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged TauD in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taurine + 2-oxoglutarate + O2 Escherichia coli
-
sulfite + aminoacetaldehyde + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged TauD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
taurine + 2-oxoglutarate + O2
-
Escherichia coli sulfite + aminoacetaldehyde + succinate + CO2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Escherichia coli

General Information

General Information Comment Organism
additional information development of a colorimetric assay method, based on the measurement of sulfite using Ellman's reagent, i.e. 2.5 mM 5,5'-dithiobis(2-nitrobenzoic acid), for determination of taurine in commercially available beverages and some biological samples, overview Escherichia coli