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Literature summary for 1.14.11.17 extracted from

  • Kahnert, A.; Kertesz, M.A.
    Characterization of a sulfur-regulated oxygenative alkylsulfatase from Pseudomonas putida S-313 (2000), J. Biol. Chem., 275, 31661-31667.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate required for activity Pseudomonas putida
ascorbate 3fold increase of activity at 0.2 mM Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.1 mM EDTA abolishes activity completely Pseudomonas putida
additional information no reduction in enzyme activity is observed for sulfate concentrations up to 10 mM Pseudomonas putida
nonyl sulfate inhibits at concentrations between 1 and 10 mM Pseudomonas putida
SDS inhibits at concentrations between 1 and 10 mM Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity, highest activity in the presence of 0.1 mM Pseudomonas putida
additional information Ni2+, Co2+, Mn2+, Cu2+, Zn2+, Mg2+, and Ca2+ have no stimulatory effect Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30250
-
4 * 30250, gel filtration Pseudomonas putida
32000
-
4 * 32000, SDS-PAGE Pseudomonas putida
121000
-
gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida S-313
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource-Q anion-exchange column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hexyl sulfate + 2-oxoglutarate + O2
-
Pseudomonas putida hexanal + sulfite + succinate + CO2
-
?
hexyl sulfate + 2-oxoglutarate + O2
-
Pseudomonas putida S-313 hexanal + sulfite + succinate + CO2
-
?
additional information the AtsK enzyme is not involved in the utilization of taurine as a sulfur source Pseudomonas putida ?
-
?
additional information the AtsK enzyme is not involved in the utilization of taurine as a sulfur source Pseudomonas putida S-313 ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 32000, SDS-PAGE Pseudomonas putida
homotetramer 4 * 30250, gel filtration Pseudomonas putida

Synonyms

Synonyms Comment Organism
alpha-ketoglutarate-dependent dioxygenase
-
Pseudomonas putida
AtsK
-
Pseudomonas putida
oxygenative alkylsulfatase
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
in Tris-acetate buffer Pseudomonas putida