Cloned (Comment) | Organism |
---|---|
reporter plasmid: promoter region of GA2ox7 amplified from genomic DNA by PCR, fused to luciferase gene in pUC19, effector plasmid: replacing GUS gene of pBI221 with DDF1 coding region, reference plasmid with Renilla luciferase gene, introduction of the plasmids to leaves via particle bombardment, leaves incubated on 0.8% agar plates at 22°C for 24 h. transgenic plants: PCR-amplificatoin of DDF1 coding region, cloned into pENTER-TOPO, inserted into vector pGWB8, Agrobacterium (strain EHA105)-mediated transformation of wild-type and GA2ox7-2 plants | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | loss-of-funtion mutation of GA2ox7 suppresses the dwarf phenotype of DWARF AND DELAYED FLOWERING 1 (DDF1) mutants, indicating that their gibberellin deficiency is due to overexpression of GA2ox7, DDF1 activates the promoter of GA2ox7 in leaves. Under high-salinity stress (250 mM), osmotic stress (300 mM mannitol) GA2ox7 is unregulated and slightly unregulated under the stress hormone abscisic acid (100 microM), the GA2ox7 loss-of-function mutant is less growth retarded than the wild-type under high-salinity stress | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin + 2-oxoglutarate + O2 | Arabidopsis thaliana | gibberellin 12 and 53 | 2beta-hydroxygibberellin + succinate + CO2 | gibberellin 110, the product of gibberellin 12 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
wild-type Columbia (Col), DWARF AND DELAYED FLOWERING 1 (DDF1) overexpressing mutant, loss-of-funtion mutants ddf1-LF1 and ga20x7-2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin + 2-oxoglutarate + O2 | gibberellin 12 and 53 | Arabidopsis thaliana | 2beta-hydroxygibberellin + succinate + CO2 | gibberellin 110, the product of gibberellin 12 | ? |
Synonyms | Comment | Organism |
---|---|---|
C20-gibberellin deactivation enzyme | - |
Arabidopsis thaliana |
GA 2-oxidase 7 | - |
Arabidopsis thaliana |
GA2ox7 | - |
Arabidopsis thaliana |
gibberellin 2-oxidase | - |
Arabidopsis thaliana |