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Literature summary for 1.13.12.7 extracted from

  • Woodroofe, C.C.; Meisenheimer, P.L.; Klaubert, D.H.; Kovic, Y.; Rosenberg, J.C.; Behney, C.E.; Southworth, T.L.; Branchini, B.R.
    Novel heterocyclic analogues of firefly luciferin (2012), Biochemistry, 51, 9807-9813.
    View publication on PubMed
Search for more literature for 1.13.12.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 (4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.028
-
 D-luciferin pH 8.2, temperature not specified in the publication Photinus pyralis
0.061
-
 (4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 9.1, temperature not specified in the publication Photinus pyralis
0.07
-
 (4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.176
-
 (4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2 decay time to 10%: 500 sec, relative specific activity: 13% Photinus pyralis ?
-
 ?
(4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2 decay time to 10%: 150 sec, relative specific activity: 1.7% Photinus pyralis ?
-
 ?
(4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2 decay time to 10%: 5700 sec, relative specific activity: 421% Photinus pyralis ?
-
 ?
(4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2 decay time to 10%: 400 sec, relative specific activity: 0.4% Photinus pyralis ?
-
 ?
(4S)-2-(6-hydroxy-1H-indol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid + ATP + O2
-
 Photinus pyralis ?
-
 ?
D-luciferin + ATP + O2 decay time to 10%: 23 sec, relative specific activity: 100% Photinus pyralis oxidized D-luciferin + AMP + CO2 + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
luciferin
-
 Photinus pyralis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00051
-
 (4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.00063
-
 (4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.0018
-
 (4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.0067
-
 (4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 9.1, temperature not specified in the publication Photinus pyralis
0.19
-
 D-luciferin pH 8.2, temperature not specified in the publication Photinus pyralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 substrate: D-luciferin Photinus pyralis
8.5
-
 substrate: (4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid Photinus pyralis
8.5
-
 substrate: (4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid Photinus pyralis
8.5
-
 substrate: (4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid Photinus pyralis
9.1
-
 substrate: (4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid Photinus pyralis

pH Range

pH Minimum pH Maximum Comment Organism
8.2 9.1
-
 Photinus pyralis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 (4S)-2-(6-hydroxy-1,3-benzoxazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.01
-
 (4S)-2-(6-hydroxy-1-benzofuran-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.03
-
 (4S)-2-(6-hydroxy-1H-benzimidazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 8.5, temperature not specified in the publication Photinus pyralis
0.11
-
 (4S)-2-(6-hydroxy-1-benzothiophen-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid pH 9.1, temperature not specified in the publication Photinus pyralis
6.78
-
 D-luciferin pH 8.2, temperature not specified in the publication Photinus pyralis