Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Lampyris turkestanicus |
Protein Variants | Comment | Organism |
---|---|---|
E353Q/ins356R | emission of red and green light at pH 7.8, 20% less activity | Lampyris turkestanicus |
E354K | emission of red and green light at pH 7.8, 26% higher activity | Lampyris turkestanicus |
E354K/ins356R | emission of red and green light at pH 7.8, 20% higher activity | Lampyris turkestanicus |
E354R | emission of red and green light at pH 7.8, 33% higher activity | Lampyris turkestanicus |
E354R/ins356R | emission of red light at pH 7.8 compared to green emitted light by wild-type luciferase, 53% higher activity compared to wild-type luciferase | Lampyris turkestanicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
D-luciferin | mutant E354K/ins356K | Lampyris turkestanicus | |
0.012 | - |
D-luciferin | mutant E354Q/ins356K | Lampyris turkestanicus | |
0.013 | - |
D-luciferin | mutant E354K | Lampyris turkestanicus | |
0.014 | - |
D-luciferin | mutant E354R/ins356K | Lampyris turkestanicus | |
0.016 | - |
D-luciferin | mutant E354R | Lampyris turkestanicus | |
0.023 | - |
D-luciferin | wild-type luciferase | Lampyris turkestanicus | |
0.04 | - |
ATP | mutant E354K/ins356K | Lampyris turkestanicus | |
0.05 | - |
ATP | mutant E354Q/ins356K | Lampyris turkestanicus | |
0.063 | - |
ATP | mutant E354K | Lampyris turkestanicus | |
0.071 | - |
ATP | wild-type luciferase | Lampyris turkestanicus | |
0.095 | - |
ATP | mutant E354R/ins356K | Lampyris turkestanicus | |
0.115 | - |
ATP | mutant E354R | Lampyris turkestanicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lampyris turkestanicus | Q5UFR2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA sepharose, 95% purity | Lampyris turkestanicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
12000000000 relative light units/s/mg, mutant E354Q/ins356K | Lampyris turkestanicus |
additional information | - |
15000000000 relative light units/s/mg, wild-type luciferase | Lampyris turkestanicus |
additional information | - |
18000000000 relative light units/s/mg, mutant E354K/ins356K | Lampyris turkestanicus |
additional information | - |
19000000000 relative light units/s/mg, mutant E354K | Lampyris turkestanicus |
additional information | - |
20000000000 relative light units/s/mg, mutant E354R | Lampyris turkestanicus |
additional information | - |
23000000000 relative light units/s/mg, mutnat E354R/ins356K | Lampyris turkestanicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + ATP + O2 | - |
Lampyris turkestanicus | oxidized luciferin + AMP + CO2 + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Lampyris turkestanicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lampyris turkestanicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | depending on mutation | Lampyris turkestanicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
mutant E354R, E354K, E354R/ins356Arg with greater thermostability than wild-type luciferase | Lampyris turkestanicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Lampyris turkestanicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | depending on mutation | Lampyris turkestanicus |