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Literature summary for 1.13.12.7 extracted from

  • Said Alipour, B.; Hosseinkhani, S.; Ardestani, S.K.; Moradi, A.
    The effective role of positive charge saturation in bioluminescence color and thermostability of firefly luciferase (2009), Photochem. Photobiol. Sci., 8, 847-855.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Lampyris turkestanicus

Protein Variants

Protein Variants Comment Organism
E353Q/ins356R emission of red and green light at pH 7.8, 20% less activity Lampyris turkestanicus
E354K emission of red and green light at pH 7.8, 26% higher activity Lampyris turkestanicus
E354K/ins356R emission of red and green light at pH 7.8, 20% higher activity Lampyris turkestanicus
E354R emission of red and green light at pH 7.8, 33% higher activity Lampyris turkestanicus
E354R/ins356R emission of red light at pH 7.8 compared to green emitted light by wild-type luciferase, 53% higher activity compared to wild-type luciferase Lampyris turkestanicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
D-luciferin mutant E354K/ins356K Lampyris turkestanicus
0.012
-
D-luciferin mutant E354Q/ins356K Lampyris turkestanicus
0.013
-
D-luciferin mutant E354K Lampyris turkestanicus
0.014
-
D-luciferin mutant E354R/ins356K Lampyris turkestanicus
0.016
-
D-luciferin mutant E354R Lampyris turkestanicus
0.023
-
D-luciferin wild-type luciferase Lampyris turkestanicus
0.04
-
ATP mutant E354K/ins356K Lampyris turkestanicus
0.05
-
ATP mutant E354Q/ins356K Lampyris turkestanicus
0.063
-
ATP mutant E354K Lampyris turkestanicus
0.071
-
ATP wild-type luciferase Lampyris turkestanicus
0.095
-
ATP mutant E354R/ins356K Lampyris turkestanicus
0.115
-
ATP mutant E354R Lampyris turkestanicus

Organism

Organism UniProt Comment Textmining
Lampyris turkestanicus Q5UFR2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA sepharose, 95% purity Lampyris turkestanicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
12000000000 relative light units/s/mg, mutant E354Q/ins356K Lampyris turkestanicus
additional information
-
15000000000 relative light units/s/mg, wild-type luciferase Lampyris turkestanicus
additional information
-
18000000000 relative light units/s/mg, mutant E354K/ins356K Lampyris turkestanicus
additional information
-
19000000000 relative light units/s/mg, mutant E354K Lampyris turkestanicus
additional information
-
20000000000 relative light units/s/mg, mutant E354R Lampyris turkestanicus
additional information
-
23000000000 relative light units/s/mg, mutnat E354R/ins356K Lampyris turkestanicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + ATP + O2
-
Lampyris turkestanicus oxidized luciferin + AMP + CO2 + diphosphate + hv
-
?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
Lampyris turkestanicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Lampyris turkestanicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 35 depending on mutation Lampyris turkestanicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
32
-
mutant E354R, E354K, E354R/ins356Arg with greater thermostability than wild-type luciferase Lampyris turkestanicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Lampyris turkestanicus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 depending on mutation Lampyris turkestanicus