Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains XL1-Blue and BL21 | Photinus pyralis |
expressed in Escherichia coli strains XL1-Blue and BL21 | Lampyris turkestanicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
D-luciferin | at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 | Lampyris turkestanicus | |
0.03 | - |
D-luciferin | at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 | Photinus pyralis | |
0.0955 | - |
ATP | in 50 mM Tricine-NaOH buffer with 10 mM MgSO4, at pH 7.8 | Lampyris turkestanicus | |
0.14 | - |
ATP | at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 | Photinus pyralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM) | Photinus pyralis | |
Mg2+ | the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM) | Lampyris turkestanicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lampyris turkestanicus | Q5UFR2 | - |
- |
Photinus pyralis | Q27758 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose column chromatography | Photinus pyralis |
Ni-NTA Sepharose column chromatography | Lampyris turkestanicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + O2 + ATP | - |
Photinus pyralis | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? | |
D-luciferin + O2 + ATP | - |
Lampyris turkestanicus | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Photinus pyralis |
firefly luciferase | - |
Lampyris turkestanicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Photinus pyralis |
25 | - |
- |
Lampyris turkestanicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | activity starts to decrease above 25°C and is almost completely lost at 45°C | Photinus pyralis |
25 | 45 | activity starts to decrease above 25°C and is almost completely lost at 45°C | Lampyris turkestanicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Photinus pyralis |
8.5 | - |
- |
Lampyris turkestanicus |