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Literature summary for 1.13.12.7 extracted from

  • Mortazavi, M.; Hosseinkhani, S.; Khajeh, K.; Ranjbar, B.; Emamzadeh, A.R.
    Spectroscopic and functional characterization of Lampyris turkestanicus luciferase: a comparative study (2008), Acta Biochim. Biophys. Sin. (Shanghai), 40, 365-374.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains XL1-Blue and BL21 Photinus pyralis
expressed in Escherichia coli strains XL1-Blue and BL21 Lampyris turkestanicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
D-luciferin at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 Lampyris turkestanicus
0.03
-
D-luciferin at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 Photinus pyralis
0.0955
-
ATP in 50 mM Tricine-NaOH buffer with 10 mM MgSO4, at pH 7.8 Lampyris turkestanicus
0.14
-
ATP at pH 7.8, in 50 mM Tricine-NaOH buffer with 10 mM MgSO4 Photinus pyralis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM) Photinus pyralis
Mg2+ the enzyme catalyzes the oxidation of substrate luciferin in the presence of Mg2+ (10 mM) Lampyris turkestanicus

Organism

Organism UniProt Comment Textmining
Lampyris turkestanicus Q5UFR2
-
-
Photinus pyralis Q27758
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose column chromatography Photinus pyralis
Ni-NTA Sepharose column chromatography Lampyris turkestanicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-luciferin + O2 + ATP
-
Photinus pyralis oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?
D-luciferin + O2 + ATP
-
Lampyris turkestanicus oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv
-
?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
Photinus pyralis
firefly luciferase
-
Lampyris turkestanicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Photinus pyralis
25
-
-
Lampyris turkestanicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 45 activity starts to decrease above 25°C and is almost completely lost at 45°C Photinus pyralis
25 45 activity starts to decrease above 25°C and is almost completely lost at 45°C Lampyris turkestanicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Photinus pyralis
8.5
-
-
Lampyris turkestanicus