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Literature summary for 1.13.12.7 extracted from

  • Kitayama, A.; Yoshizaki, H.; Ohmiya, Y.; Ueda, H.; Nagamune, T.
    Creation of a thermostable firefly luciferase with pH-insensitive luminescent color (2003), Photochem. Photobiol., 77, 333-338.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E356R/V368A mutant enzyme with significantly improved thermostability, more than 90% of the activity remains after incubation for 1 h at 45°C. Unlike the wild-type enzyme the mutant enzyme shows no change in the emission maximum of 568 nm even at pH 6.3 Luciola parvula

Organism

Organism UniProt Comment Textmining
Luciola parvula
-
-
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
1 h, less than 10% loss of activity mutant enzyme E356R/V368A Luciola parvula