Literature summary for 1.13.12.7 extracted from

Zako, T.; Ayabe, K.; Aburatani, T.; Kamiya, N.; Kitayama, A.; Ueda, H.; Nagamune, T.
Luminescent and substrate binding activities of firefly luciferase N-terminal domain (2003), Biochim. Biophys. Acta, 1649, 183-189.

Search for more literature for 1.13.12.7

Activating Compound

Activating Compound	Comment	Organism	Structure
CoA	enhances luminescent activity of the wild-type enzyme about 2fold, highest activity at 1.2 mM CoA. For the N-terminal domain the effect of CoA is modest, 1.1fold enhancement, highest activity at 0.01 mM CoA. Addition of CoA at higher concentrations inhibits the luminescence activity	Photinus pyralis

Cloned(Commentary)

Cloned (Comment)	Organism
Nn-terminal domain, expression in Escherichia coli	Photinus pyralis

Protein Variants

Protein Variants	Comment	Organism
DELTA438-550	the purified N-terminal domain 1-437 has luminescence activity by itself, and binds to substrates ATP and luciferin with reduced affinity	Photinus pyralis

Inhibitors

Inhibitors	Comment	Organism	Structure
CoA	above 0.1 mM, N-terminal domain	Photinus pyralis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0072	-	Luciferin	wild-type enzyme	Photinus pyralis
0.026	-	Luciferin	N-terminal domain	Photinus pyralis
0.23	-	ATP	wild-type enzyme	Photinus pyralis
6.9	-	ATP	N-terminal domain	Photinus pyralis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	optimal concentration for wild-type enzyme and N-terminal domain: 10 mM	Photinus pyralis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	x * 49000, N-terminal domain, SDS-PAGE	Photinus pyralis
61200	-	x * 61200, wild-type enzyme, SDS-PAGE	Photinus pyralis

Organism

Organism	UniProt	Comment	Textmining
Photinus pyralis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
luciferin + O2 + ATP	-	Photinus pyralis	oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv	-	?

Subunits

Subunits	Comment	Organism
?	x * 49000, N-terminal domain, SDS-PAGE	Photinus pyralis
?	x * 61200, wild-type enzyme, SDS-PAGE	Photinus pyralis

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Release 2023.1 (January 2023)