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Literature summary for 1.13.12.7 extracted from

  • Thompson, J.F.; Geoghegan, K.F.; Lloyd, D.B.; Lanzetti, A.J.; Magyar, R.A.; Anderson, S.M.; Branchini, B.R.
    Mutation of protease-sensitive region in firefly luciferase alters light emission properties (1997), J. Biol. Chem., 272, 18766-18771.
    View publication on PubMed
Search for more literature for 1.13.12.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Photinus pyralis

Organism

Organism UniProt Comment Textmining
Photinus pyralis P08659
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutants purified using ammonium sulfate precipitation and Sephacryl S-200 column chromatography Photinus pyralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 relative specific activity is 30% lower at pH 8.7 than at pH 7.8 Photinus pyralis

Storage Stability

Storage Stability Organism
5°C, pH 7.2, ammonium sulfate, EDTA, glycerol, several weeks Photinus pyralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Photinus luciferin + O2 + ATP
-
 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
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-
 Photinus pyralis