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Literature summary for 1.13.11.33 extracted from

  • Kundumani-Sridharan, V.; Dyukova, E.; Hansen, D.E.; Rao, G.N.
    12/15-Lipoxygenase mediates high-fat diet-induced endothelial tight junction disruption and monocyte transmigration a new role for 15(S)-hydroxyeicosatetraenoic acid in endothelial cell dysfunction (2013), J. Biol. Chem., 288, 15830-15842 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonate + O2 Mus musculus
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(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?
arachidonate + O2 Mus musculus C57BL/6
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(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus P39654
-
-
Mus musculus C57BL/6 P39654
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-

Source Tissue

Source Tissue Comment Organism Textmining
aorta
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Mus musculus
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artery
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Mus musculus
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endothelium
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Mus musculus
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monocyte
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Mus musculus
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vascular smooth muscle cell
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
Mus musculus (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?
arachidonate + O2
-
Mus musculus C57BL/6 (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?

Synonyms

Synonyms Comment Organism
12/15-lipoxygenase
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Mus musculus
12/15-LO
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Mus musculus

General Information

General Information Comment Organism
malfunction deletion of 12/15-LO negates endothelial tight junctions disruption and monocyte adhesion caused by the high-fat diet Mus musculus
physiological function 12/15-lipoxygenase plays a role in atherosclerosis. 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE), the major 12/15-LO metabolite of arachidonic acid, induces endothelial barrier permeability via Src and Pyk2-dependent zonula occluden (ZO)-2 tyrosine phosphorylation and its dissociation from the tight junction complexes. 15(S)-HETE also stimulates macrophage adhesion to the endothelial monolayer in Src and Pyk2-dependent manner. Exposure of arteries from wild-type mice to arachidonate or 15(S)-HETE leads to Src-Pyk2-dependent ZO-2 tyrosine phosphorylation, tight junction disruption, and macrophage adhesion, whereas the arteries from 12/15-LO knock-out mice are protected from these effects of arachidonate. Feeding wild-type mice with a high-fat diet induces the expression of 12/15-LO in the arteries leading to tight junction disruption and macrophage adhesion, and deletion of the 12/15-LO gene disallows these effects. 15(S)-HETE-induced endothelial tight junctions disruption promotes monocyte transmigration, 15(S)-HETE induces the dislocation of both claudin-1 and claudin-5 from ZO-2, that participates in tight junctions Mus musculus