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Literature summary for 1.13.11.33 extracted from

  • Middleton, M.K.; Zukas, A.M.; Rubinstein, T.; Kinder, M.; Wilson, E.H.; Zhu, P.; Blair, I.A.; Hunter, C.A.; Pure, E.
    12/15-lipoxygenase-dependent myeloid production of interleukin-12 is essential for resistance to chronic toxoplasmosis (2009), Infect. Immun., 77, 5690-5700.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
PD146176 selective inhibitor of 12/15-LOX, macrophages treated with PD146176 elaborate reduced levels of interleukin-12 in response to Toxoplasma gondii antigen Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
C57BL/6 mice
-
Mus musculus C57BL/6
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C57BL/6 mice
-

Source Tissue

Source Tissue Comment Organism Textmining
brain 12/15-LOX is selectively enhanced in the periphery, 12/15-LOX-expressing myeloid cells are enriched in the brain during chronic toxoplasmosis Mus musculus
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macrophage 12/15-LOX is a macrophage-selective regulator of interleukin-12 production Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
Mus musculus (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?
arachidonate + O2
-
Mus musculus C57BL/6 (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?

Synonyms

Synonyms Comment Organism
12/15-lipoxygenase
-
Mus musculus
12/15-LOX
-
Mus musculus

Expression

Organism Comment Expression
Mus musculus 12/15-LOX activity is enhanced during chronic, but not acute, toxoplasmosis up

General Information

General Information Comment Organism
physiological function 12/15-LOX is a critical mediator of the chronic type 1 inflammatory response. Evolution of the immune response to Toxoplasma gondii is accompanied by an increasing requirement for 12/15-LOX mediated signaling. Although 12/15-LOX deficient mice are resistant to acute Toxoplasma gondii infection, 80% of 12/15-LOX-deficient mice die during chronic toxoplasmosis, compared to no deaths in wild-type controls. The enhanced susceptibility of 12/15-LOX-deficient mice to chronic toxoplasmosis is associated with reduced production of IL-12 and gamma interferon (IFN-gamma) that is not evident during acute infection. Ex vivo IFN-gamma production by 12/15-LOX-deficient splenocytes can be rescued by the addition of recombinant IL-12. 12/15-LOX does not play a role in macrophage killing of Toxoplasma gondii in vitro Mus musculus