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Literature summary for 1.13.11.33 extracted from

  • Wecksler, A.T.; Kenyon, V.; Garcia, N.K.; Deschamps, J.D.; van der Donk, W.A.; Holman, T.R.
    Kinetic and structural investigations of the allosteric site in human epithelial 15-lipoxygenase-2 (2009), Biochemistry, 48, 8721-8730.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.33

Activating Compound

Activating Compound Comment Organism Structure
13-(S)-hydroxyoctadecadienoic acid allosteric effector, which changes the conformation of 15-hLO-2 such that substrate affinity toward linoleic acid is significantly increased Homo sapiens

Application

Application Comment Organism
medicine pro-tumorigenic feedback loop for 13-(S)-hydroxyoctadecadienoic acid against 15-hLO-2 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
without a His6-tag Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
linoleic acid fully deuterated, the mode of inhibition, in the presence of 13-(S)-hydroxyoctadecadienoic acid, changes from mixed-type inhibition to competitive inhibition Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe iron-containing metalloenzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
without a His6-tag, more than 90% pure Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
epithelium
-
 Homo sapiens
-
prostate
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
 Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
 ?

Synonyms

Synonyms Comment Organism
15-hLO-2
-
 Homo sapiens
15-lipoxygenase-2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.57
-
 arachidonic acid at 15°C, normalized to iron content Homo sapiens
0.62
-
 arachidonic acid at pH 7 Homo sapiens
0.74
-
 arachidonic acid at pH 8 Homo sapiens
0.75
-
 arachidonic acid at pH 7.5 Homo sapiens
0.75
-
 arachidonic acid at 22°C, normalized to iron content Homo sapiens
1.04
-
 arachidonic acid at 30°C, normalized to iron content Homo sapiens
1.38
-
 arachidonic acid at 37°C, normalized to iron content Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 8 approximately level between pH 7 and 8 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
 linoleic acid fully deuterated, in the presence of 13-(S)-hydroxyoctadecadienoic acid Homo sapiens
0.017
-
 linoleic acid fully deuterated Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00005
-
 arachidonic acid at 15°C, normalized to iron content Homo sapiens
0.00015
-
 arachidonic acid at 22°C, normalized to iron content Homo sapiens
0.00015
-
 arachidonic acid at pH 7.5 Homo sapiens
0.00024
-
 arachidonic acid at pH 7 Homo sapiens
0.00028
-
 arachidonic acid at pH 8 Homo sapiens
0.00046
-
 arachidonic acid at 30°C, normalized to iron content Homo sapiens
0.00075
-
 arachidonic acid at 37°C, normalized to iron content Homo sapiens