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Literature summary for 1.13.11.31 extracted from

  • Ivanov, I.; Di Venere, A.; Horn, T.; Scheerer, P.; Nicolai, E.; Stehling, S.; Richter, C.; Skrzypczak-Jankun, E.; Mei, G.; Maccarrone, M.; Kuehn, H.
    Tight association of N-terminal and catalytic subunits of rabbit 12/15-lipoxygenase is important for protein stability and catalytic activity (2011), Biochim. Biophys. Acta, 1811, 1001-1010.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
Y98A kcat and Km (linoleic acid) decreased compared to wild-type, mutant shows strongly reduced catalytic activity compared to wild-type Oryctolagus cuniculus
Y98F kcat and Km (linoleic acid) increased compared to wild-type Oryctolagus cuniculus
Y98R mutant shows strongly reduced catalytic activity compared to wild-type, mutant does not follow Michaelis-Menten-kinetics. Mutant is strongly inhibited by linoleic acid at concentrations above 0.01 mM Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
linoleic acid pH 7.4, 20°, mutant Y98A Oryctolagus cuniculus
0.0169
-
linoleic acid pH 7.4, 20°, wild-type Oryctolagus cuniculus
0.0213
-
linoleic acid pH 7.4, 20°, mutant Y98F Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid + O2
-
Oryctolagus cuniculus ?
-
?

Synonyms

Synonyms Comment Organism
12/15-lipoxygenases
-
Oryctolagus cuniculus
12/15-LOX
-
Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.7
-
linoleic acid pH 7.4, 20°, mutant Y98A Oryctolagus cuniculus
44.2
-
linoleic acid pH 7.4, 20°, wild-type Oryctolagus cuniculus
65.2
-
linoleic acid pH 7.4, 20°, mutant Y98F Oryctolagus cuniculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1170
-
linoleic acid pH 7.4, 20°, mutant Y98A Oryctolagus cuniculus
2600
-
linoleic acid pH 7.4, 20°, wild-type Oryctolagus cuniculus
3060
-
linoleic acid pH 7.4, 20°, mutant Y98F Oryctolagus cuniculus