Activating Compound | Comment | Organism | Structure |
---|---|---|---|
13-hydroperoxy-octadecadienoic acid | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed with hexa-His tags | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
11-thialinoleic acid | is a competitive inhibitor of 12-lipoxygenase with arachidonate as substrate. Presence of inhibitor does not alter the product distribution for 12-lipoxygenase. It does not change the regioselectivity of 12-lipoxygenase | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by one step Ni2+ affinity chromatography, more than 90% pure | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood platelet | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-80°C, 10-20% glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12-lipoxygenase | - |
Homo sapiens |
12/15-LO | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
11-thialinoleic acid | - |
Homo sapiens |