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Literature summary for 1.12.7.2 extracted from

  • Soboh, B.; Linder, D.; Hedderich, R.
    A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis (2004), Microbiology, 150, 2451-2463.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
reduced ferredoxin 70°C Caldanaerobacter subterraneus subsp. tengcongensis
0.015
-
oxidized ferredoxin 70°C Caldanaerobacter subterraneus subsp. tengcongensis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane bound Caldanaerobacter subterraneus subsp. tengcongensis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe NiFe hydrogenase Caldanaerobacter subterraneus subsp. tengcongensis
Ni NiFe hydrogenase, contains 0.9 mol Ni per mol enzyme Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14700
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
17000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
32000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
40000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis
69000
-
x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H+ + reduced ferredoxin Caldanaerobacter subterraneus subsp. tengcongensis
-
H2 + oxidized ferredoxin
-
r

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-sepharose HiLoad, hydroxyapatite, Superdex 200 Caldanaerobacter subterraneus subsp. tengcongensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
38.5
-
reduction of metronidiazole Caldanaerobacter subterraneus subsp. tengcongensis
391
-
reduction of methyl viologen Caldanaerobacter subterraneus subsp. tengcongensis
1260
-
oxidation of methyl viologen Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+ + reduced ferredoxin
-
Caldanaerobacter subterraneus subsp. tengcongensis H2 + oxidized ferredoxin
-
r
H+ + reduced methyl viologen
-
Caldanaerobacter subterraneus subsp. tengcongensis H2 + oxidized methyl viologen
-
r
H2 + ferredoxin + oxidized metronidazole
-
Caldanaerobacter subterraneus subsp. tengcongensis H+ + ferredoxin + reduced metronidazole
-
?

Subunits

Subunits Comment Organism
? x * 69000 + x * 40000, + x * 32000, + x * 17000 + x + 14100 + x * 14700, SDS-PAGE Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

Synonyms Comment Organism
Ech hydrogenase
-
Caldanaerobacter subterraneus subsp. tengcongensis