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Literature summary for 1.12.7.2 extracted from

  • Van Dijk, C.; Mayhew, S.G.; Grande, H.J.; Veeger, C.
    Purification and properties of hydrogenase from Megasphaera elsdenii (1979), Eur. J. Biochem., 102, 317-330.
    View publication on PubMed
Search for more literature for 1.12.7.2

Activating Compound

Activating Compound Comment Organism Structure
MgCl2 up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation Megasphaera elsdenii
NaCl up to 0.5 M, activation of methyl or benzyl viologen mediated hydrogen oxidation Megasphaera elsdenii
Tris-HCl at pH 8, activation of methyl or benzyl viologen mediated hydrogen oxidation Megasphaera elsdenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.7
-
 Oxidized benzyl viologen hydrogen oxidation Megasphaera elsdenii
171
-
 oxidized methyl viologen hydrogen oxidation Megasphaera elsdenii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration Megasphaera elsdenii
52000
-
 SDS-PAGE Megasphaera elsdenii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + oxidized ferredoxin Megasphaera elsdenii
-
 H+ + reduced ferredoxin
-
 r
H2 + oxidized ferredoxin Megasphaera elsdenii LC1
-
 H+ + reduced ferredoxin
-
 r

Organism

Organism UniProt Comment Textmining
Megasphaera elsdenii
-
 formerly Peptostreptococcus elsdenii
-
Megasphaera elsdenii LC1
-
 formerly Peptostreptococcus elsdenii
-

Purification (Commentary)

Purification (Comment) Organism
-
 Megasphaera elsdenii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
510
-
 reaction with methyl viologen Megasphaera elsdenii
1470
-
 after addition of 1 M NaCl, reaction with methyl viologen Megasphaera elsdenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + electron acceptor methyl viologen as electron acceptor Megasphaera elsdenii H+ + reduced electron acceptor
-
 r
H2 + electron acceptor benzyl viologen as electron acceptor Megasphaera elsdenii H+ + reduced electron acceptor
-
 r
H2 + electron acceptor methyl viologen as electron acceptor Megasphaera elsdenii LC1 H+ + reduced electron acceptor
-
 r
H2 + electron acceptor benzyl viologen as electron acceptor Megasphaera elsdenii LC1 H+ + reduced electron acceptor
-
 r
H2 + oxidized benzyl viologen
-
 Megasphaera elsdenii H+ + reduced benzyl viologen
-
 r
H2 + oxidized benzyl viologen
-
 Megasphaera elsdenii LC1 H+ + reduced benzyl viologen
-
 r
H2 + oxidized ferredoxin
-
 Megasphaera elsdenii H+ + reduced ferredoxin
-
 r
H2 + oxidized ferredoxin
-
 Megasphaera elsdenii LC1 H+ + reduced ferredoxin
-
 r
H2 + oxidized methyl viologen
-
 Megasphaera elsdenii H+ + reduced methyl viologen
-
 r
H2 + oxidized methyl viologen
-
 Megasphaera elsdenii LC1 H+ + reduced methyl viologen
-
 r