Literature summary for 1.12.7.2 extracted from

Fischer, J.; Quentmeier, A.; Kostka, S.; Kraft, R.; Friedrich, C.G.
Purification and characterization of the hydrogenase from Thiobacillus ferrooxidans (1996), Arch. Microbiol., 165, 289-296.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	6.02 mol per mol enzyme	Acidithiobacillus ferrooxidans
Ni	0.72 mol per mol enzyme	Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34000	-	1 * 64000 + 1 * 34000, SDS-PAGE	Acidithiobacillus ferrooxidans
64000	-	1 * 64000 + 1 * 34000, SDS-PAGE	Acidithiobacillus ferrooxidans
100000	-	native PAGE	Acidithiobacillus ferrooxidans

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	-	-
Acidithiobacillus ferrooxidans ATC 19859	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.02	-	hydrogen oxidation, methylene blue as electron acceptor	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + electron acceptor	ferricyanide as electron acceptor	Acidithiobacillus ferrooxidans	H+ + reduced electron acceptor	-	r
H2 + electron acceptor	methylene blue as electron acceptor	Acidithiobacillus ferrooxidans	H+ + reduced electron acceptor	-	r
H2 + electron acceptor	ferricyanide as electron acceptor	Acidithiobacillus ferrooxidans ATC 19859	H+ + reduced electron acceptor	-	r
H2 + electron acceptor	methylene blue as electron acceptor	Acidithiobacillus ferrooxidans ATC 19859	H+ + reduced electron acceptor	-	r

Subunits

Subunits	Comment	Organism
dimer	1 * 64000 + 1 * 34000, SDS-PAGE	Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
49	-	-	Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)