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Literature summary for 1.12.7.2 extracted from
- Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus (1989), J. Biol. Chem., 264, 5070-5079.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O2 | reversible inhibition | Pyrococcus furiosus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 31 atoms per mol | Pyrococcus furiosus | |
| Ni | 0.98 atoms per mol enzyme | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 27000 | - |
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 46000 | - |
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 185000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized ferredoxin | Pyrococcus furiosus | - |
H+ + reduced ferredoxin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
strain DSM 3638 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + electron acceptor | methylene blue as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
| H2 + electron acceptor | methyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
| H2 + oxidized ferredoxin | - |
Pyrococcus furiosus | H+ + reduced ferredoxin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
hydrogen evolution | Pyrococcus furiosus |
| 95 | - |
above 95°C, hydrogen oxidation | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
most of activity is retained after 1 h incubation | Pyrococcus furiosus |
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