Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | H2-production from reduced methyl viologen requires phospholipids | Desulfovibrio vulgaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | - |
Desulfovibrio vulgaris | - |
- |
cytoplasmic membrane | of lactate/sulfate grown cells. The enzyme is present in the cell in two states: the majority is membrane-associated (the [NiFeSe]m form) and a minor amount is soluble (the [NiFeSe]s form) | Desulfovibrio vulgaris | - |
- |
soluble | of lactate/sulfate grown cells. The enzyme is present in the cell in two states: the majority is membrane-associated (the [NiFeSe]m form) and a minor amount is soluble (the [NiFeSe]s form) | Desulfovibrio vulgaris | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | NiFeSe enzyme | Desulfovibrio vulgaris | |
Fe | [NiFeSe] enzyme, ratio Ni:Se:Fe is 1:1:15 | Desulfovibrio vulgaris | |
Ni | NiFeSe enzyme, contains selenium in equimolar amounts to Ni | Desulfovibrio vulgaris | |
Ni | [NiFeSe] enzyme, ratio Ni:Se:Fe is 1:1:15 | Desulfovibrio vulgaris | |
Se | NiFeSe enzyme, contains selenium in equimolar amounts to Ni | Desulfovibrio vulgaris | |
Se | [NiFeSe] enzyme, ratio Ni:Se:Fe is 1:1:15 | Desulfovibrio vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 63000 + x * 35000, SDS-PAGE | Desulfovibrio vulgaris |
63000 | - |
x * 63000 + x * 35000, SDS-PAGE | Desulfovibrio vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + ferricytochrome c3 | Desulfovibrio vulgaris | - |
H+ + ferrocytochrome c3 | - |
? | |
H2 + ferricytochrome c3 | Desulfovibrio vulgaris Hildenborough | - |
H+ + ferrocytochrome c3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio vulgaris | - |
- |
- |
Desulfovibrio vulgaris | - |
Hildenborough | - |
Desulfovibrio vulgaris Hildenborough | - |
- |
- |
Desulfovibrio vulgaris Hildenborough | - |
Hildenborough | - |
Oxidation Stability | Organism |
---|---|
resistance of the [NiFeSe] Hase to inactivation by oxygen | Desulfovibrio vulgaris |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | post-translationally modified, probably by the binding of a lipidic group at the N-terminus. The presence of such a group explains the membrane association of the enzyme, the requirement of phospholipids for maximal activity, and the blockage of the large subunit to N-terminal sequencing | Desulfovibrio vulgaris |
Purification (Comment) | Organism |
---|---|
- |
Desulfovibrio vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:lactate/sulfate-grown cell | - |
Desulfovibrio vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + ferrocytochrome c3 | - |
Desulfovibrio vulgaris | H2 + ferricytochrome c3 | - |
? | |
H+ + ferrocytochrome c3 | - |
Desulfovibrio vulgaris Hildenborough | H2 + ferricytochrome c3 | - |
? | |
H+ + reduced methyl viologen | - |
Desulfovibrio vulgaris | H2 + methyl viologen | - |
? | |
H+ + reduced methyl viologen | - |
Desulfovibrio vulgaris Hildenborough | H2 + methyl viologen | - |
? | |
H2 + ferricytochrome c | - |
Desulfovibrio vulgaris | H+ + ferrocytochrome c | - |
? | |
H2 + ferricytochrome c | - |
Desulfovibrio vulgaris Hildenborough | H+ + ferrocytochrome c | - |
? | |
H2 + ferricytochrome c3 | - |
Desulfovibrio vulgaris | H+ + ferrocytochrome c3 | - |
? | |
H2 + ferricytochrome c3 | - |
Desulfovibrio vulgaris Hildenborough | H+ + ferrocytochrome c3 | - |
? | |
reduced methyl viologen + H+ | - |
Desulfovibrio vulgaris | oxidized methyl viologen + H2 | - |
? | |
reduced methyl viologen + H+ | - |
Desulfovibrio vulgaris Hildenborough | oxidized methyl viologen + H2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000 + x * 35000, SDS-PAGE | Desulfovibrio vulgaris |
Synonyms | Comment | Organism |
---|---|---|
[NiFeSe] Hase | - |
Desulfovibrio vulgaris |
[NiFeSe] hydrogenase | - |
Desulfovibrio vulgaris |