KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
mutant cytochrome c3 Y65A | 25°C, pH 7.0, mutant cytochrome c3 Y65A | Desulfovibrio vulgaris | |
0.0026 | - |
ferricytochrome c | 25°C, pH 7.0, wild-type cytochrome c3 | Desulfovibrio vulgaris | |
0.0027 | - |
mutant cytochrome c3 K10M | 25°C, pH 7.0, mutant cytochrome c3 K10M | Desulfovibrio vulgaris | |
0.003 | - |
mutant cytochrome c3 K26M | 25°C, pH 7.0, mutant cytochrome c3 K26M | Desulfovibrio vulgaris | |
0.0043 | - |
mutant cytochrome c3 Y66L | 25°C, pH 7.0, mutant cytochrome c3 Y66L | Desulfovibrio vulgaris | |
0.0045 | - |
mutant cytochrome c3 K15M | 25°C, pH 7.0, mutant cytochrome c3 K15M | Desulfovibrio vulgaris | |
0.0051 | - |
mutant cytochrome c3 K57M | 25°C, pH 7.0, mutant cytochrome c3 K57M | Desulfovibrio vulgaris | |
0.0057 | - |
mutant cytochrome c3 K58M | 25°C, pH 7.0, mutant cytochrome c3 K58M | Desulfovibrio vulgaris | |
0.0057 | - |
mutant cytochrome c3 K94M | 25°C, pH 7.0, mutant cytochrome c3 K94M | Desulfovibrio vulgaris | |
0.0067 | - |
mutant cytochrome c3 K72M | 25°C, pH 7.0, mutant cytochrome c3 K72M | Desulfovibrio vulgaris | |
0.0073 | - |
mutant cytochrome c3 K95M | 25°C, pH 7.0, mutant cytochrome c3 K95M | Desulfovibrio vulgaris | |
0.0099 | - |
mutant cytochrome c3 K101M | 25°C, pH 7.0, mutant cytochrome c3 K101M | Desulfovibrio vulgaris | |
0.0104 | - |
mutant cytochrome c3 K60M | 25°C, pH 7.0, mutant cytochrome c3 K60M | Desulfovibrio vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio vulgaris | - |
- |
- |
Desulfovibrio vulgaris Miyazaki F | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + ferricytochrome c | - |
Desulfovibrio vulgaris | H+ + ferrocytochrome c | - |
? | |
H2 + ferricytochrome c | - |
Desulfovibrio vulgaris Miyazaki F | H+ + ferrocytochrome c | - |
? | |
H2 + ferricytochrome c3 | the positive charges of Lys60, Lys72, Lys95, and Lys101 around heme 4 are important for formation of the transient complex with [NiFe] hydrogenase in the initial stage of the cytochrome c3 reduction. Reaction with wild-type and mutant cytochrome c3 | Desulfovibrio vulgaris | H+ + ferrocytochrome c3 | - |
? | |
H2 + ferricytochrome c3 | the positive charges of Lys60, Lys72, Lys95, and Lys101 around heme 4 are important for formation of the transient complex with [NiFe] hydrogenase in the initial stage of the cytochrome c3 reduction. Reaction with wild-type and mutant cytochrome c3 | Desulfovibrio vulgaris Miyazaki F | H+ + ferrocytochrome c3 | - |
? | |
H2 + mutant cytochrome c K101M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K15M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K26M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K57M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K58M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K60M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K72M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K94M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c K95M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c Y65A | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c Y66L | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K101M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K101M | - |
Desulfovibrio vulgaris Miyazaki F | ? | - |
r | |
H2 + mutant cytochrome c3 K10M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K10M | - |
Desulfovibrio vulgaris Miyazaki F | ? | - |
r | |
H2 + mutant cytochrome c3 K15M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K15M | - |
Desulfovibrio vulgaris Miyazaki F | ? | - |
r | |
H2 + mutant cytochrome c3 K26M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K57M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K58M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K60M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K72M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K94M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 K95M | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 Y65A | - |
Desulfovibrio vulgaris | ? | - |
r | |
H2 + mutant cytochrome c3 Y66L | - |
Desulfovibrio vulgaris | ? | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
mutant cytochrome c K72M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
44 | - |
mutant cytochrome c K26M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
45 | - |
mutant cytochrome c K15M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
45 | - |
mutant cytochrome c K58M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
45 | - |
mutant cytochrome c K94M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
45 | - |
mutant cytochrome c K95M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
46 | - |
mutant cytochrome c K60M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
47 | - |
mutant cytochrome c Y66L | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
48 | - |
mutant cytochrome c K57M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
48 | - |
mutant cytochrome c3 K10M | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
49 | - |
mutant cytochrome c Y65A | 25°C, pH 7.0 | Desulfovibrio vulgaris | |
49 | - |
ferricytochrome c | 25°C, pH 7.0, wild-type cytochrome c3 | Desulfovibrio vulgaris | |
51 | - |
mutant cytochrome c K101M | 25°C, pH 7.0 | Desulfovibrio vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Desulfovibrio vulgaris |