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Literature summary for 1.12.2.1 extracted from

  • Yagi, T.; Honya, M.; Tamiya, N.
    Purification and properties of hydrogenases of different origins (1968), Biochim. Biophys. Acta, 153, 699-705.
    View publication on PubMed
Search for more literature for 1.12.2.1

Inhibitors

Inhibitors Comment Organism Structure
CO
-
 Desulfovibrio desulfuricans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 ferrocytochrome c3 H2-evolution reaction Desulfovibrio desulfuricans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Desulfovibrio desulfuricans

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desulfovibrio desulfuricans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+ + reduced methyl viologen
-
 Desulfovibrio desulfuricans H2 + methyl viologen
-
 ?
H2 + ferricytochrome c3
-
 Desulfovibrio desulfuricans H+ + ferrocytochrome c3
-
 r
additional information enzyme catalyzes production of H2 from Na2S2O4 in presence of cytochrome c3 Desulfovibrio desulfuricans ?
-
 ?
additional information enzyme can catalyze H-2H exchange in absence of added electron carriers Desulfovibrio desulfuricans ?
-
 ?
additional information no reduction of ferredoxin, methylene blue or hexacyanoferrate(II) by H2 Desulfovibrio desulfuricans ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, H2 evolution in presence of methyl viologen, no decrease in activity Desulfovibrio desulfuricans
60
-
 10 min, H2 evolution in presence of methyl viologen, 30% loss of activity Desulfovibrio desulfuricans
70
-
 10 min, H2 evolution in presence of methyl viologen, 37% loss of activity Desulfovibrio desulfuricans
80
-
 10 min, H2 evolution in presence of methyl viologen, 69% loss of activity Desulfovibrio desulfuricans
90
-
 10 min, H2 evolution in presence of methyl viologen, 99% loss of activity Desulfovibrio desulfuricans
100
-
 10 min, H2 evolution in presence of methyl viologen, complete loss of activity Desulfovibrio desulfuricans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 cytochrome c3-dependent H2 evolution Desulfovibrio desulfuricans
6 7 methyl viologen-dependent H2 evolution Desulfovibrio desulfuricans
6 8 H-2H exchange Desulfovibrio desulfuricans
8 9 cytochrome c3 reduction by H2 Desulfovibrio desulfuricans

Cofactor

Cofactor Comment Organism Structure
cytochrome c3
-
 Desulfovibrio desulfuricans