Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.6 extracted from

  • Kimoto, H.; Yoshimune, K.; Matsuyma, H.; Yumoto, I.
    Characterization of catalase from psychrotolerant Psychrobacter piscatorii T-3 exhibiting high catalase activity (2012), Int. J. Mol. Sci., 13, 1733-1746.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
75
-
H2O2 pH 7.0, 25°C Psychrobacter piscatorii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
4 * 59000, SDS-PAGE Psychrobacter piscatorii
247000
-
gel filtration Psychrobacter piscatorii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 H2O2 Psychrobacter piscatorii
-
O2 + 2 H2O
-
?
2 H2O2 Psychrobacter piscatorii T-3
-
O2 + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Psychrobacter piscatorii A0A0T6DN91 isolated from the drain pool of a plant that uses H2O2 as a bleaching agent
-
Psychrobacter piscatorii T-3 A0A0T6DN91 isolated from the drain pool of a plant that uses H2O2 as a bleaching agent
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 11fold by anion exchange and hydrophobic chromatography, followed by ultrafiltration Psychrobacter piscatorii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19700
-
native enzyme, cell extract, pH 7.0, 25°C Psychrobacter piscatorii
222000
-
purified native enzyme, pH 7.0, 25°C Psychrobacter piscatorii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
Psychrobacter piscatorii O2 + 2 H2O
-
?
2 H2O2
-
Psychrobacter piscatorii T-3 O2 + 2 H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 59000, SDS-PAGE Psychrobacter piscatorii

Synonyms

Synonyms Comment Organism
PktA
-
Psychrobacter piscatorii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Psychrobacter piscatorii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 10 broad optimum Psychrobacter piscatorii

pH Range

pH Minimum pH Maximum Comment Organism
3 12 activity range, 53.4% of maximal activity at pH 4.0, 18.4% at pH 3.0 Psychrobacter piscatorii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
purified native enzyme, 15 min, 60°C, complete inactivation Psychrobacter piscatorii
8
-
purified native enzyme, 15 min, 60°C, most stable at Psychrobacter piscatorii
11
-
purified native enzyme, 15 min, 60°C, complete inactivation Psychrobacter piscatorii

Cofactor

Cofactor Comment Organism Structure
heme binding sites of the distal region of heme with Val106, Thr128 and Phe143, and proximal sites of heme with Tyr348 and Arg355 are well conserved Psychrobacter piscatorii
additional information the enzyme contains a NADPH-binding site with His184, Arg193, Val292 and Lys295 Psychrobacter piscatorii

General Information

General Information Comment Organism
evolution the dismutation reaction of H2O2 in microorganisms has evolved in three phylogenetically unrelated protein types: monofunctional catalase, catalase-peroxidase and Mn-catalase, phylogenetic analysis, overview. PktA is a clade 3 catalase. The active sites with His65, Ser104, and Asn138, binding sites of the distal region of heme with Val106, Thr128, and Phe143, and proximal sites of heme with Tyr348 and Arg355 are well conserved Psychrobacter piscatorii
additional information the enzyme exhibits an extraordinarily high catalase activity, active sites residues are His65, Ser104, and Asn138 Psychrobacter piscatorii