Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization and kinetics of catalase on calcium carbonate nanoparticles attached epoxy support, synthesized by miniemulsion technique, overview. A decrease in Vmax value from 1.50 to 0.42 mM/mg protein is observed after immobilization. Thermal and storage stabilities of catalase improved immensely after immobilization. The immobilized enzyme retains three times than the activity of free enzyme when kept at 75°C for 1 h and the half-life of enzyme increases five times when stored in 0.01 M phosphate, pH 7.0, at 5°C. The enzyme can be reused 30times without any significant loss of its initial activity | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.73 | - |
H2O2 | pH 7.0, 25°C, free enzyme | Aspergillus niger | |
31.42 | - |
H2O2 | pH 7.0, 25°C, immobilized enzyme | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Aspergillus niger | - |
O2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus niger | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Aspergillus niger | O2 + 2 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
free enzyme and immobilized enzyme on nanoparticles | Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 75 | activity range, free enzyme and immobilized enzyme on nanoparticles | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
free enzyme and immobilized enzyme on nanoparticles | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range, free enzyme and immobilized enzyme on nanoparticles | Aspergillus niger |